Low and Superlow Fields and Radiation in Medicine and Biology

International Congress since 1997

LSFMB 8, 2018

ULTRASTRUCTURAL ANALYSIS OF BLOOD CELLS AT LIGHT-OXYGEN AND PHOTODYNAMIC EFFECTS

Alekseev Yu.V., Barkhina T.G.1, Davydov E.V.2, Mislavsky O.V.3, Gushchin M.Yu.3, Ivanov A.V.4

State Scientific Center of Laser Medicine of FMBA of Russia, Moscow, e-mail: [email protected] Institute of Human Morphology”, Moscow, [email protected]

2Veterinary clinic “Veles-Tekstilschiki”, Moscow, [email protected]
3State Scientific Center «Institute of Immunology» FMBA of Russia, Moscow4N.N.Blokhin National Medical Research Center of Oncology, Moscow, [email protected]

Study of blood cells with the help of transmission (TEM) and scanning (SEM) electron microscopy under the influence of light-oxygen and photodynamic effects. Preliminary data suggest that the light-oxygen and photodynamic effects have a specific effect on blood cells, which is a prerequisite for determining the effectiveness of using each method in clinical practice.

Литература

1. S. D. Zakharov, A. V. Ivanov Light–Oxygen Effect as a Physical Mechanism for Activation of Biosystems by Quasi- Monochromatic Light (A Review) // Biophysics, 2005, Vol. 50, Suppl. 1, pp. S64–S85.
2. S D Zakharov, AV Ivanov Light-oxygen effect in cells and its potential applications in tumour therapy // Quantum Electronics 1999, vol/29 (12), pp. 1031 – 1053.

3. Алексеев Ю.В., Захаров С.Д., Иванов А.В. Фотодинамический и светокислородный эффекты: общность и различия// Лазерная медицина. 2012, т.16, вып. 4, с.40-46.

ACTIONS OF BIOLOGICAL ACTIVE SUBSTANCES TO BIOSYSTEMS

Alekseeva, O.M., Krementsova, A.V., Krivandin, A.V. Golochshapov A N., Kim Yu.A. 1

Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow, Russia [email protected] of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, [email protected]

This work deals with the actions of biological active substances (BAS) to the cellular and model bio-objects of animal origin. The BAS are melafen – plant growth regulator, fenozan and its derivatives – antioxidants with different molecular structures, by these derivatives have variable properties when influence to membrane and cellular experimental objects. The objects are multilammelar liposomes, formed from synthetic or natural phospholipids, erythrocytes, cells of Erlich ascetic carcinoma and lymphocytes ant thymocytes. We obtained data, which suggest that BAS influenced to structure of synthetic liposomes, but not to natural. To the structure and functions of cells BAS acted too.

Литература

1. Тараховский Ю.С., Кузнецова С.М., Васильева Н.А., Егорочкин М.А., Ким Ю.А. Взаимодействие таксифолина (дигидрокверцитина) с мультиламеллярными липосомами из димиристоилфосфатидилхолина // Биофизика. 2008. Т. 53. No 1. С. 78-84.
2. Алексеева О.М., Кривандин А.В., Шаталова О.В., Рыков В.А., Фаттахов С.Г., Бурлакова Е.Б., Коновалов А.И. Исследование взаимодействия мелафена с фосфолипидными мембранами. // Доклады академии наук. 2009. Т. 427. No 6. С. 837-839.

3. Векшина О.М. (Алексеева), Фаткуллина Л.Д., Ким Ю.А., Бурлакова Е.Б.. «Изменения структуры и функций мембран эритроцитов и клеток асцитной карциномы Эрлиха при действии гибридного антиоксиданта нового поколения ИХФАН-10» // Бюллетень экспериментальной биологии и медицины. 2007. No 4. С. 402-406.
4. Бинюков В. И., Алексеева О. М., Миль Е. М., Албантова А.А., Фаттахов С. Г., Голощапов А. Н., Бурлакова Е. Б., Коновалов А. И. Изучение влияния фенозана, ИХФАН-10 и мелафена на эритроциты in vivo методом АСМ. // Доклады академии наук 2011. Т. 441. No 1. С.114-117.

СHEMILUMINESCENCE ANALYSISOF DIHYDROQUERCETIN ANTIOXIDANT PROPERTIES

Babenkova I.V., Buravlev E.A.1, Teselkin Yu.O.

Pirogov Russian National Research Medical University, E-mail: [email protected] First Moscow State Medical University (Sechenov University), E-mail: [email protected]

The development of new drugs based on natural compounds from the flavonoid family is an actual goal. Dihydroquercetin is one of the promising bioflavonoids. However, the antioxidant mechanisms of dihydroquercetin are still far from full understanding.

Three chemiluminescence model systems were used to study antioxidant activity of dihydroquercetin: by decreasing of Fe(II)-depended peroxide oxidation of liposome lipids (system 1), by inhibition of luminol oxidation induced by 2,2′-azobis(2-methylpropionamidine) dihydrochloride (system 2) or by opsonized zymosan-stimulated polymorphonuclear leukocytes (system 3). Dihydroquercetin increased the chemiluminescence latent period in system 1 (concentration 0.1−2.5 μM) and system 2 (concentration 0.05−0.15 μM). In system 1, the antioxidant effect ofdihydroquercetin did not differ from the water-soluble trolox. In system 2, where water-soluble peroxide radicals were generated, the antioxidant activity of dihydroquercetinwas 6 times greater than that of the trolox. In system 3, dihydroquercetin reduced the intensity of luminol-dependent chemiluminescence of polymorphonuclear leukocytes at concentrations of 5−125 μM (cell count–1.5×105/ml).

Thus, complex chemiluminescence analysis allowed to evaluate antioxidant activity of dihydroquercetin and to reveal some features of its antioxidant mechanism.

PHYSICO-CHEMICAL FEATURES OF HIGHLY DILUTE WATER SYSTEMS, DUE TO THE PRESENCE OF ATMOSPHERIC GASES

L.V. Belovolova, M.V. Glushkov

General Physics Institute. A.M. Prokhorov of the Russian Academy of Sciences, Vavilov St., 38, Moscow, Russia; e-mail: [email protected]

The fluorescence (λex = 260 nm), Rayleigh light scattering (λex = λobs. = 260 nm), electrical conductivity, redox potential and response to ultraviolet irradiation of purified water and simultaneously prepared samples of the 15th centennial dilution of sodium chloride, potassium chloride and acetone have been investigated. It was found that immediately after preparation and at least for the next two days these samples significantly differ from each other and from the initial water. Using the methods of flicker-noise spectroscopy, the kinetic dependences of the light scattering of these systems and their variation during time have been studied. It can be concluded that the serial dilution and shaking of aqueous

solutions leads to increasing of content of reactive oxygen species and to intensification of their reactions with impurities and dissolved atmospheric gases.

INFLUENCE OF DIFFERENT ORIENTATION OF ALTERNATING ELECTRIC FIELD RELATIVE VERTICAL AND HORIZONTAL STATIC MAGNETIC FIELDS ON GRAVITROPJSM

Bogatina N.I., Sheykina N.V.1, Linnik A.S.

B. Verkin Institute for Low Temperature Physics &Engineering of National Academy of Sciences of Ukraine, Kharkov, Ukraine. E-mail: [email protected]

1 –National University of Pharmacy, Kharkov, Ukraine, E-mail: [email protected]

Gravitropic reaction of cress roots relatively static vertical and horizontal magnetic fields combined with alternating electric field tuned to the cyclotron frequency of Ca2+ions was investigated for all possible relative orientations of fields and roots’ directions. It was shown that that thegravitropic reaction depends essentially on the relative orientation of magnetic, electric fields and roots direction. Two mechanisms were proposed to explain this effect. The first one is based on the taking in account the expansion of the directed ions flow in magnetic field and breathing of the membrane with the frequency of alternating magnetic field. The secondone is based on the possibility of phase transition in water. For the last mechanism thereare no obstacles for the ion cyclotron existence because in that case ions rotate around the domains of water in vacuum between the domains.

RESEARCH OF OWN MICROWAVE RADIATION OF THE HUMAN BRAIN

Leonid I.Brusilovsky, Andrey S. Bryukhovetskiy1

ELBRUS Corporation LTD, Moscow, Russia, [email protected]
1Closed Joint-Stock Company “Clinic of Interventional Neurology and Rehabilitation Therapy” Neurovita “, Moscow,

Russia, [email protected]

The report presents studies and experimental evidence of the existence of the own electromagnetic activity of the human brain in the UHF and SHF rages from 1.5 to 4.5 GHz with signal power at the level of -130 dBm..-110 dBm (1е-16….1е-14 W). The method of registration will be the basis for the development of a fundamentally new technology of microwave encephalography (Patent Application No. 2017126117 of July 20, 2017).

Литература

1. Брюховецкий А.С. Проблемы теоретической неврологии. Информационно-коммутативное устройство и принципы работы мозга человека. -М.:Изд. Полиграф-Плюс, 2014 .- 330 с: 44 ил
2. Andrey S. Bryukhovetskiy Human Brain Theory. Information-Commutation Device of Brain and Principles of its Work and Modeling .-New York .-2016,Nova Science Publishers.-220p

BICARBONATE AQUEOUS SOLUTION SAND NATURAL DRINKING WATERS AS TEST SYSTEMS ON THE ACTIVITY OFF ACTORS OF ULTRA-WEAK INTENSITY

Buravleva E.V., Yablonskaya O.I.1, VoeikovV.L.

Biological Faculty,M.V. LomonosovMSU, 1N.M. EmanuelIBCPRAS,Moscow, Russia,

[email protected]

Bicarbonate aqueous systems represent sustained non-equilibrium system in which processes with the participation of active oxygen species accompanied under specific conditions by weak photon emission continuously proceed. It is demonstrated that the parameters of photon emission may be influenced by weak Cosmo physical fields and also by addition to these aqueous systems of extremely high diluted preparations of different biologically active compounds.

Литература

1. Воейков В.Л. и др. Устойчиво неравновесное состояние бикарбонатных водных систем // Ж. физ. химии, 2012, том 86, No 9, с. 1518-1527.
2. AndrievskyG.V. etal. On the production of an aqueous colloidal solution of fullerenes // J. Chem. Soc. Chem.Commun., 1995, V. 4, No 12, P. 1281-1282.

REDOX POTENTIAL KINETICS OF THE INCUBATION MEDIUM AS A REFLECTION OF THE FUNCTIONAL FEATURES OF THE EARLY DEVELOPMENT STAGES OF LOACH MISGURNUS FOSSILIS

Burlakov A.B., Kuzmin V.I.1, Gadzaov A.F.1, Tytik D.L.1, Kasatkin V.E.1, and Sleptsova L.A.

Department of Biology, Moscow State University, Moscow, Russia, [email protected]
1Moscow Technological University (MIREA), Moscow, Russia
2Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, 119991 Russia

One of the most important characteristics of the embryonic development of organisms, particularly fish, is the duration of one mitotic cycle, the period of synchronous cleavage divisions (τ0). Having such an internal rhythm in the developing embryo, it is natural to expect the manifestation of a number of other rhythmic processes connected, for example, with the metabolic processes individual for each stage of development. These processes can be investigated, because the processes, occurring within the developing organism at different stages are closely related with the changing of physicochemical properties of the incubation environment, for example, by measuring its oxidation- reduction potential (ORP).

It was revealed that the geometric progression of the moments when the critical development periods comes during synchronization with mitotic cycles determines the positions of the lines having an elevated rank of significance. It is shown that the sequence of critical boundaries (critical periods of development) is represented by the following values: 3.16τ0, 8.6τ0, 23.4τ0, 63.5τ0, 173.5τ0, 470τ0. These values, like their half, are well manifested in the kinetic of the incubation medium ORP changes up to 130τ0. The other times can be used, possibly, as predictive assessments of the

critical period boundaries in the development of the

Литература

1. Бурлаков А.Б., Кузьмин В.И., Гадзаов А.Ф., Тытик Д.Л., Касаткин В.Э., Слепцова Л.А. Кинетика окислительно-восстановительного потенциала среды инкубации в период раннего развития вьюна Misgurnus fossilis // Онтогенез, 2017, том 48, No 1, с. 28–38.
2. Кузьмин В.И., Гадзаов А.Ф., Бурлаков А.Б., Тытик Д.Л., Касаткин В.Э., Бусев С.А. Моделирование критических периодов развития вьюна Misgurnus fossilis по кинетике изменения окислительно-восстановительного потенциала среды инкубации на основе пропорции Кирквуда // Технологии живых систем,том 15, No 2, с. 30-39.

IMPACT OF MILLIMETER ELECTROMAGNETIC WAVES ON BLOOD PLASMA BUFFER CAPACITY OF RATS
Vardevanyan P.O., Nerkararyan A.V., Shahinyan M.A., Mikaelyan M.S.
Department of Biophysics, Faculty of Biology, Yerevan State University, Yerevan, Armenia, A. Manoogian 1, 0025,[email protected]

The impact of millimeter range electromagnetic waves (MM EMW) on rat blood plasma buffer capacity has been studied. It was shown that MM EMW results in decreasing of plasma buffer capacity. Thus, for the reduction of plasma pH by a unit it is necessary 4 ml of 0.1 N HCl solution. In the case of irradiation it is needed only 0.09 ml HCl to change the plasma pH by a unit. Most probably, it is connected to the alterations taking place in the plasma proteins, after which they lose their ability to catch protons and the value of pH rapidly changes.

Литература

1. Rifat F., Saxena V.K., Srivastava P., Sharma A., Sisodia R. Effects of 10 GHz MW exposure on hematological changes in Swiss albino mice and their modulation by Prunus domestica fruit extract. // Intern. J. of Advanced Res., 2014, v. 2, N2, p. 386-396.
2. Ongel K., Gumral N., Ozguner F. The potential effect of electromagnetic field: A review. // Cell membrane and free radical research, 2009, N1, p. 85-89.

3. Hood, E. Electromagnetic field and DNA effects: Potential mechanism elucidated. // Environmental Health perspectives, 2001, v. 112(A), p. 368.
4. Shahinyan M.A., Antonyan A.P., Mikaelyan M.S., Vardevanyan P.O. Study of influence of millimeter range electromagnetic waves on water-saline solutions of albumin. // Biophysical reviews and letters, 2015, v. 10, N4, p. 20. 5. Vardevanyan P.O., Antonyan A.P., Shahinyan M.A., Mikaelyan M.S. // Influence of millimeter electromagnetic waves on fluorescence of water-saline solutions of human serum albumin. // J. of Appl. Spectr., 2016, v. 83, N3, p. 486. 6. Shckorbatov Y.G., Grigoryeva N.N., Shakhbazov V.G., Grabina V.A., Bogoslavsky A.M. Microwave irradiation influences on the state of human cell nuclei. // Bioelectromagnetics, 1998, v. 19, p. 414-419.

INFLUENCE OF A WEAK STATIC MAGNETIC FIELD ON COGNITIVE FUNCTIONS IN RECIPROCAL HYBRIDS OF DROSOPHILA MELANOGASTER

Vasileva S.A., Nikitina E.A.1, Medvedeva A.V., Savvateeva-Popova E.V.,
Shchegolev B.F.2, Surma S.V., Stefanov V.E.3
Pavlov institute of physiology RAS, St. Petersburg, Russia, [email protected], [email protected],

[email protected], [email protected], [email protected]
1Herzen State Pedagogical University, St. Petersburg, Russia, [email protected] National Medical Research Centre, St. Petersburg, Russia, [email protected]
3St. Petersburg State University, Biochemistry Department, St. Petersburg, Russia, [email protected]

Drosophila mutant agnts3 of agnostic locus which encompass LIMK1 gene is an adequate model to study the epigenetic regulation of genome organization, being involved in regulation of actin remodeling cascade. Lately, the genome parent-of-origin effects are considered to be particularly important for prognostic models construction in predictive medicine. In this study, we have investigated the parent effect of agnts3 mutation in training and memory formation. Our data let us to conclude that locus limk1 is among the factors parentally affecting behavior.

Литература

1. Горохова С.А., Никитина Е.А. и др. Воздействие ослабленного в космическом пространстве магнитного поля Земли на транскрипционную активность генома, обучение и память // Тезисы XII Международной конференции «Пилотируемые полеты в космос». Россия, Звездный городок. 2017. С. 87 – 89;
2. Никитина Е.А., Медведева А.В и др. Ослабленное магнитное поле Земли: влияние на транскрипционную активность генома, обучение и память у Dr. melanogaster // Ж. высшей нервной деятельности им. И.П. Павлова. 2017. Т. 67. No 2. С. 246–256.

THE INFLUENCE OF MILLIMETER WAVES ELECTROMAGNETIC RADIATION ON THE GROWTH OF MAMMALIAN CELL CULTURES
Velikanov A.N., Karelin S.A., Tambiev A.H.
Lomonosov Moscow State University, Biological Faculty, Moscow, Russia, e-mail: [email protected],

Electromagnetic radiation of millimeter range (also extremely high frequency, EHF 30-300 GHz) was reproduced in artificial conditions only in 1965. A number of interesting effects were found during the study of the effect of EHF on biological objects and this range of radiation began to be used in medicine [1, 2]. Сhanges in the growth rate of cell cultures under the action of millimeter radiation were shown [3, 4, 5], also specific action of different irradiation modes [6].The current subjects of interest are the mechanismsunderlying the observed biological effects of millimeter waves.

Литература

1. Девятков, Н.Д., Голант, М.Б., Бецкий, О.В. Миллиметровые волны и их роль в процессах жизнедеятельности. М., Радио и связь – 1991 – 169 с.
2. Тамбиев А.Х., Кирикова, Н.Н., Бецкий, О.В., Гуляев, Ю.В. / Миллиметровые волны и фотосинтезирующие организмы. // Издательский дом “Радиотехника” – 2003 – 175 с.

3. Тамбиев, А.Х., Баграташвили, В.Н., Герасимов, Ю.В., Свиридов, А.П., Антонов, Е.Н., Чайлахян, Р.К. Действие электромагнитного излучения миллиметрового диапазона низкой интенсивности на стволовые стромальныеклетки костного мозга. // Материалы XV Междунар. конф. “Новые информационные технологии в медицине, биологии, фармакологии и экологии” – 2007 – с. 56-57.

4. Chailakhyan, R.K., Yusupov, V.I., Gorskaya, Yu.F., Kuralesova, A.I., Gerasimov, Yu.V., Sviridov, A.P., Tambiev, A.Kh., Vorob’eva, N.N., Grosheva, A.G., Shishkova, V.V., Moskvina, I.L., Bagratashvili, V.N. Effects of Acoustic and EHF Impulses on Multipotent Stromal Cells during Formation of Bone Marrow Containing Heterotopic Organs in Tissue Engineered Constructions. // Bull Exp Bio Med – 2015 – 158 (5) – p. 688-691.

5. Великанов А.Н., Петрова Л.А., Супруненко Е.А., Тамбиев А.Х., Бурлаков А.Б. Влияние непрерывного и импульсного электромагнитного излучения КВЧ диапазона на культуры клеток млекопитающих// труды VII Международного конгресса “Слабые и сверхслабые поля и излучения в биологии и медицине”, 2015, с. 24-25.
6. Гапеев, А.Б. Исследование механизмов биологического действия низкоинтенсивного электромагнитного излучения крайне высоких частот: успехи, проблемы, перспективы. // Биомедицинская радиоэлектроника – 2014 – 6 – с. 20-30.

EFFECTS OF PLANT PEPTIDES ON BIOLOGICAL AND PHYSICAL-CHEMICAL TEST SYSTEMS IN A WIDE CONCENTRATION RANGE
Voeikov V.L., BuravlevaE.V., SkripnikovA.Yu.
Biological Faculty, M.V. Lomonosov MSU, Moscow, Russia [email protected]

Plant peptides belonging to CLE family in a wide range of concentrations including ultra-low ones effect the balance between growth and differentiation in roots of Arabidopsis thaliana seedlings and intensity of the processes with reactive oxygen species participation permanently proceeding in human blood and bicarbonate aqueous solutions. Concentration dependence of the effects is non-monotonous, hormesis-like. These and other features of their action argue against the traditional “lock-and-key” mechanism for peptide action.

Литература

1. Kucukoglu M., Nilsson O. CLE peptide signaling in plants – the power of moving around //Physiol Plant.,2015, V. 155, No 1, p 74–87.
2. Tsukagoshi H. et al.Transcriptional regulation of ROScontrols transition from proliferation to differentiation in the root. // Cell, 2010, V.143, No 4, p 606–616.

3. Voeikov V.L., et al. Biophoton research in blood reveals its holistic properties// Indian J. Exp. Biol., 2003, V.43, No 5, p. 473-482.
4. ВоейковВ.Л. и др. Устойчиво неравновесное состояние бикарбонатных водных систем // Ж. физ. химии, 2012, том 86, No 9, с. 1518-1527.

TO THE MEMORY OF LEV BELOUSSOV THE MISTERY OF THE EMBRYO DEVELOPMENT

I.V. Volodyaev

Biological faculty of M.V. Lomonosov Moscow State University, Moscow, Russia E-mail: [email protected]

Embryo development is a process of incomparable complication, both morphogenetic (formation of space structures), and functional (differentiation of cell lines). Amazingly, most (if not all) of its aspects belong to the type of self-organizational processes, and have natural parallels in physics of open nonequilibrium systems and mathematics of dynamical systems. Yet, unlike ordinary “selforganization in the flow” some of them take place at the “relaxation branch” of system dynamics, and demand a kind of “sensing of the whole”.

In yearslong works of Lev Beloussov and coauthors, morphogenesis and cell movements in embryos were shown largely regulated by patterns of mechanical tension and strain in it. Moreover, the “sensing of whole” mystery was fully explained by mechanical feedbacks in the tissues and their property to actively react to external mechanical strain and hyper restore it.

Embryo development has two seemingly incompatible aspects: determinism of the whole process appearing in the obligate succession of key stages, and variability of patterns of gene expression, cell movements and morphodynamics observed in certain periods. Being associated with spontaneous decrease of symmetry in many aspects (space, dynamic and “cell-fate”), embryogenesis appears a permanent entwinement of structurally stable dynamics (deterministic aspect) and symmetry-breaking events (associated with irreducible variability and quasi-stochastic processes).

EXPERIENCE – THE SON OF MISTAKES: A CRITICAL REVIEW EXPERIMENTAL STUDIES OF MITOGENETIC EFFECT

I.V. Volodyaev, E.V. Naumova1

Biological faculty of M.V. Lomonosov Moscow State University, Moscow, Russia1Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia
E-mail: [email protected]

Mitogenetic effect – is acceleration of cell divisions in microbe and cell cultures, and tissues, caused by distant (optical) contact with a number of biological and chemical systems. MGE was first discovered in [1], and was further investigated in 1920-s—1940-s by a number of scientific groups in the USSR, the USA, Germany, Italy, France, and other countries [2]. The total number of publications on the topic is more than 700, including a number of articles in top-rating journals [8]. Yet, the effect was not widely appreciated in the end, as a number of influential authors failed to reproduce it in their works [5].

Detailed analysis of methodical aspects of these works reveal severe violation of the methods suggested in the “positive works” [8]. Here we critically review the main methodical aspects of both “positive” and “negative” works on MGE and try to state recommendations for its reproducible registration. We consider MGE an unresolved problem in both fundamental [6] and applied biology [7], that should be revisited with the use of presepnt-day techniques, knowledge and level of evidence. Anyway, further progress in this area is impossible without rigorous investigation of earlier works with a particular focus on methods and evidence.

Литература

1. Gurwitsch A.G. Die Natur des spezifischen Erregers der Zellteilung // Arch. Mikrosk. Anat. und Entw. Mech. 1923.No 100. P. 11–40.
2. Rahn O. Invisible radiations of organisms. Berlin: Gebruder Borntraeger, 1936.
3. Wolf L.K., Ras G. Einige Untersuchungen uber die mitogenetischen Strahlen von Gurwitsch // Centr. Bact. I.Orig. 1931. Vol. 123. P. 257.

4. Tuthill J.B., Rahn. O. Zum Nachweis mitogenetischer Strahlung durch Hefesprossung // Arch. f. Mikrobiol. 1933. Vol. 4. P. 565–573.
5. Hollaender A., Claus W.D. An experimental study of the problem of mitogenetic radiation // Bulletin of the Nat. Research Council. Washington: National research council of the National academy of sciences, 1937.

6. Gurwitsch A.G., Gurwitsch L.D. Die mitogenetische Strahlung. Jena: Fischer Verlag, 1959.
7. Pesochensky B.S. Quenching of mitogenetic radiation of blood in cancer and precancerous deseases // Collected volume on mitogenesis and the theory of biological field. Moscow: Pub.house of the USSR Academy of Medical Sciences, 1947. P. 102–114.
8. Volodyaev I., Beloussov L. V. Revisiting the mitogenetic effect of ultra-weak photon emission. // Front. Physiol. 2015. Vol. 6. P. 241.

THE MECHANISMS OF RADIATION PROTECTIVE EFFECT OF PULSE-MODULATED EXTREMELY HIGH-FREQUENCY ELECTROMAGNETIC RADIATION

Gapeyev A.B., Lukyanova N.A.

Institute of Cell Biophysics of Russian Acad. Sci., Pushchino, Moscow region, 142290, Russia, e-mail: [email protected]

Using the comet assay technique, it was shown that low-intensity pulse-modulated electromagnetic radiation of extremely high frequencies (EHF EMR) with certain physical parameters can cause a radiation protective effect, protecting cellular DNA from x-ray radiation. The effect depended on the sequence of actions, frequency and type of modulation, intensity and carrier frequency. The mechanisms of radiation protective effect are associated with the induction of an adaptive response by nanomolar concentrations of reactive oxygen species formed by the pulse- modulated radiation. Cessation of the radiation protective effect of EHF EMR with selective inhibition of SAPK/JNK kinase cascade and non-specific blocking of DNA repair systems indicates the important role of these intracellular systems in the realization of the mechanisms of radiation protective action of low-intensity EHF EMR.

INVESTIGATION OF THE EFFECT OF AGENTS WITH ANTIOXIDANT STATUS ON MICROVISCOSITY OF THE MICE BRAIN ORGANELLES MEMBRANES IN HEALTH

Gerasimov N.Yu., Nevrova O.V., Goloshchapov A.N.,

IBCP RAS
The effect of the phenosan in different concentration, from low to ultralow, was studied. Significant changes of the micrviscosity were found out. Maximal effect was observed for two concentrations of the antioxidant, that is 10-17 and 10-5 mole/kg. The drug in all concentrations reduced microviscosity of the lipidic areas and increased microviscosity of the proteinic areas of the membranes.

BIOLOGICAL ACTIVITY OF STRONGLY DILUTED SOLUTIONS OF POTASSIUM ANPHEN

Zhigacheva I.V., Volodkin A.A.

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia, tel. +7(495)939-74-09, E-mail: zhigacheva@mail, [email protected]

The antistress properties of 2-carboxy-2- (N-acetylamino) -3- (3 ‘, 5′-di-tert-butyl-4’-hydroxyphenyl –propionate potassium (potassium anphen) were investigated. To simulate stress effects, the model of “aging” mitochondria were used, which causes the activation of LPO in the membranes of these organelles [Zhigacheva I. V., Burlakova E. B., 2014]. Aging” of rat liver mitochondria and mitochondria of pea seedlings caused a weak swelling of mitochondria and an increase of fluorescence intensity of LPO products in 3-4 times. The introduction of potassium anphen into the mitochondrial incubation medium led to a decrease in the fluorescence of the final products of lipid peroxidation both in the membranes of mitochondria of rat liver and in the membranes of mitochondria of pea seedlings and had a dose dependence. The preparation in concentrations of 10-5-10-8, 10-13-10-16M reduced the fluorescence intensity of LPO products to a control level. In these concentrations, the drug increased the oxidation maximal rates of NAD-dependent substrates in the respiratory chain of plant and animal mitochondria by 13.2-35%. Based on the data obtained, it can be assumed that the biological effects of potassium anphen are due to its antioxidant properties and the ability of its highly diluted (10-16-10-6 M) aqueous solutions to spontaneously form molecular ensembles, denoted by the term “nanoassociates”. Quantum- chemical calculations of associates of potassium anphen suggest the possibility of the existence of hydrate-containing agglomerates in an aqueous medium. The reactivity of such agglomerates is associated with a change in entropy and free energy, depending on the structure of the solvation shell of the ligand.

LOW-DOSE LED IRRADIATION WITH BLUE LIGHT STIMULATS METABOLIC CELL ACTIVITY

Zack P.P*., Seryoznikova N.B.1, Pogodina L.S.1, Potashnikova D.M.1, Sakina N.L., Dontsov A.E., N.M. Emanuel

Institute of Biochemical Physics RAS, Moscow, Russia1M.V. Lomonosov Moscow State University, Moscow, Russia *E-mail: [email protected] , tel: 8 (916) 949-48-93

A study of the effect of low-dose blue LED irradiation with a maximum of 450 nm and doses of ≤1 J/cm2 was carried out on the models of RPE cells of the eye of the Japanese quail Coturnix japonica and the human monocyte cell line THP-1. Metabolic activity of cells (resazurin test), their antioxidant status (inhibition of the process of chemiluminescence of luminol induced by hydrogen peroxide) and the value of the mitochondrial membrane potential (TMRE test) were registered as measurable parameters. The results of the study on models of RPE cells and human monocytes indicate that irradiation with blue light at 450 nm in doses less than 1 J/cm2 not only does not cause damage but rather has beneficial effects on metabolic cell activity, as it was shown for irradiation by LED and laser sources in the red spectral range.

THIOL-CONTAINING PROTEINS, AS A FACTOR SUPPORTING THE TRANSPARENCY OF THE LENS UNDER CONDITIONS OF OXIDATIVE STRESS

Ibrahimova J.M, Bayramova S.D.

A.I.Karayev Institute of Physiology of Azerbaijan NAS, Baku
Тел.: +(99412) 432-33-87; e-mail: [email protected], [email protected].

The study of the effect of non-ionizing electromagnetic radiation (EMR) on the organs of vision of humans and animals remains a point of great significance over the last decade. EMR of radio and microwave frequency has become an integrated part of modern high-tech world and as a consequence, the impact of this factor on living organisms is inevitable. Recently obtained data on exposure of experimental animals to microwave radiation has pointed out the oxidative nature of EMR adverse effects. Experimental data on effect of decimeter radiation on lipid peroxidation processes and antioxidant defense in visual structures provided by Laboratory of Biophysics of Cell Metabolism of A.I. Karayev Institute of Physiology has confirmed this assumption as well. Tissue thiols inherently react with oxidizing products. Thereby the oxidative effect of non-ionizing EMR may have impact on the lens’ thiols pool.

Experiments conducted on albino rats have shown that exposure to relatively high intensity irradiation (30 μW /cm2) for 14 days led to the gradual transition of the thiol groups hidden in the protein structure to accessible (reactive) state in the cortex of the lens, and vice verse, an increase in content of hidden (inaccessible) thiols was detected in the nucleus of the lens. Low-intensity irradiation (10 μW / cm2) resulted in a decrease in accessible thiols (glutathione andsurface-located protein thiols) in the cortex (and, less obvious, in the nucleus) of the lens on background of low level of LPO. Apparently, the underlying reason for that phenomenon is the transition of protein easily accessible sulfhydryl groups to hidden form via S-glutathionylation and protein disulfide bond formation. Transition of protein thiols from one state to another attributed to physical factors impact allowed to suggest the supramolecular mechanism of regulation of homeostasis (in particular, thiols) in high-protein formations which can be realized by aggregation and de- aggregation of protein molecules.

EFFECTS OF EHF OF LOW INTENSITY FOR OXIDATIVE PROCESSES IN CELLS Kazarinov K.D., Borisenko G.G., Polnikov I.G.

Kotelnikov Institute of Radioengineering and Electronics, RAS (Fryasinobranch)

The results of experimental studies of the biological effect of microwave radiation on biochemical processes simulatind reactions in the cells in the state of oxidizing stress are presented. Here, by using model system of cytochrome c (cyt c) and phospholipidsmembranes we demonstrated that microwave irradiation can stimulatecyt c-dependent oxidation of substrates in the water and membranes. Our results suggest that microwave irradiation may elevate sensitivityof cells to apoptotic stimuli.

Литература

1. Bergstrom C.L.,Beales P.A., Lv. Y., Vanderlick T.K., Groves J.T. Cytochrome c causes pore formation in cardiolipin-containing membranes //ProcNatlAcadSci USA, 2013, V. 110(16), P. 6269-74.
2. Казаринов К., Борисенко Г.Г., Полников И.Г. Влияние ЭМИ низкой интенсивности микроволнового диапазона на окислительные процессы в клетках //Электроннаятехника. Сер. 1. СВЧ – техника, 2018, Вып.1 (536), С. 60-68.

STIMULATION OF PLANARIAN REGENERATION BY LOW COCENTRATIONS OF CERIUM OXIDE NANOPARTICLES

Kamenskikh K.A., Popov A.L., Ermakova O.N., Ermakov A.M.

Institute of Theoretical and Experimental Biophysics of RAS, 142290, Russia, Pushchino, Istitute st. 3, e-mail: [email protected]

It is shown that nanocrystalline cerium dioxide in nanomolar concentrations is capable of stimulating the rate of planarians regeneration. This effect is realized by stimulating the expression of genes responsible for regeneration and activation of stem cells mitotic activity.

Литература

1. Gentile L., Cebrià F., Bartscherer K. The planarian flatworm: an in vivo model for stem cell biology and nervous system regeneration // Disease Models & Mechanisms, 2011, V. 4, No 1, P 12-19.
2. Khaksar M.R., Rahimifard M., Baeeri M., Maqbool F., Navaei-Nigjeh M., Hassani S., Moeini-Nodeh S., Kebriaeezadeh A., Abdollahi M. Protective effects of cerium oxide and yttrium oxide nanoparticles on reduction of oxidative stress induced by sub-acute exposure to diazinon in the rat pancreas // Journal of Trace Elements in Medicine and Biology, 2017, V. 41, P. 79-90.

3. Popov A.L., Popova N.R., Selezneva I.I., Akkizov A.Y., Ivanov V.K. Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro // Mat. Sci. Eng. C., 2016, V. 68, P. 406–413.
4. Ivanov, V.K., Maksimov, V.D., Shaporev, A.S. et al. Hydrothermal synthesis of efficient TiO2-based photocatalysts // Russ. J. Inorg. Chem., 2010, V. 55, No 2, P. 154-159.

EFFECT RADIATION OF TERAHERTZ AND VISIBLE RANGES ON ERYTHROCYTES’ MEMBRANE RESISTENCE

Kolesnikov V. G., Kondakova A. K.1, Khmel N. V.
O.Ya. Usikov Institute for radiophisics and electronics of National Academy of Science of Ukraine 61085, Ukraine, Kharkov, 12 Ac. Proskura Str., E-mail: [email protected] for Dermatology and Venereology, Academy of Medical Sciences of Ukraine, 61057, Ukraine, Kharkov, 7/9 Chernishevsky Str., E-mail: [email protected]

The results of laser low-intensive terahertz radiations effects (λ= 333 mcm, Р = 6 mW/cm2, t = 30 min) and radiation of visible range (λ=0,694 mcm, Р = 12 mW/cm2, t = 30 min) on erythrocytes of donors and patients by allergic dermatosis are presented. The resistance of erythrocytes was estimated by means of hemolysis in vitro. The evaluation of influence of radiation terahertz and visible ranges was carried out with help of microwave dielectrometry (work frequency f =37,7 GHz), at that a real part of complex dielectric permeability (ε′) was measured. Results of the given researches testify to efficiency laser terahertz expositions in recovery of erythrocytes’ membrane resistance in vitro on the average 25 % that opens prospects for the further investigations of mechanisms of erythrocytes’ membranes resistance at therapy of patients by allergic dermatosis.

Литература

1. Кизилова Н.Н., Коробов А.М. Механизмы влияния низкоинтенсивного оптического излучения на систему микроциркуляции // Фотобіологія та експериментальна фотомедицина, 2016, Т.1, No 2.- С. 75-95.
2. Кондакова А. К., Семко Г.А., Древаль Н.В., Колесников В.Г., Каменев Ю.Е. Комбинированное влияние терагерцового и миллиметрового излученией на антиоксидантный статус и осмотический гемолиз эритроцитовin vitro // Дерматология и венерология, 2010, No4 (50), С. 33-37.

EXTERNAL INFLUENCES ON PREBIOTIC ORGANIC MICROSYSTEMS AS A REQUIRED FACTOR OF THEIR TRANSFORMATION INTO INITIAL FORMS OF LIFE

Kompanichenko V.N.

Institute for Complex Analysis of Regional Problems FEB RAS, Birobidzhan; [email protected]

Unlike other concepts of the origin of life, the author considers oscillations of physic-chemical parameters in the medium as a REQUIRED condition for emergence of primary microorganisms. Significant fluctuations of pressure, temperature, concentrations of components, electric potentials and other parameters in hydrothermal medium executed incessant influences upon prebiotic organic microsystems and stimulated their transformation into primary forms of life. The decisive step in this way consisted in thermodynamic inversion, after which the transformed microsystems acquired the property to continuously concentrate free energy and information as well as to export entropy. Therefore, the ability of any living system to respond upon external influences appeared just at the moment of the origin of life on early Earth.

INVESTIGATION OF THE INFLUENCE OF ULTRASOUND EXTRACTION DURATION ON EXTRACTION OF BIOLOGICALLY ACTIVE SUBSTANCES WITH ZINGIBER OFFICINALE

Kravchenko I. A.1,2, Eberle L. V. 1, Kobernik А. A. 1,2

1Department of Pharmaceutical Chemistry, I.I. Mechnikov Odessa National University
2Department of Organic and Pharmaceutical Technology of Odessa National Polytechnic University Odessa, Ukraine e-mail: [email protected] , [email protected]

The effect of conditions of polyphenol compounds extraction from the Zingiber officinale root using ultrasound (US) was investigated. It is established, that more full extraction of substances is observed at extraction by aqueous-ethanolic solvent (70 vol.% of ethanol) with method of cold maceration in US-field (frequency – 27 kHz, power of radiator – 2 W/sm2) within 15 minutes with the size of particles of raw material equal 2 mm. The offered way of extraction of vegetative extracts allows to increase efficiency and speed of phenolic compounds extraction, to lower the temperature and the time of thermal processing of an extract.

Литература

1. Kumar S., Sabiha М., Nisha R., Ankita K. Evaluation of Zingiber officinale and Curcuma longa rhizome as a crude drug from their ethanolic extract. Department of Applied Chemistry Samrat Ashok Technological Institute. Vidisha (M.P.), India. 2013; 4 (12): 74 – 76.

Abid M., Jabbar S., Wu T., Hashim M.M., et al. Ultrason Sonochem. 2014, 21(1), 93–97.
Bhat R., Kamaruddin N.S.B.Ch., Min-Tze L., Karim A.A. Ultrason Sonochem. 2011, 18(6), 1295 р.

THE DNA RESONANCE CODE MAY BE BASED ON RING CURRENTS.

Myakishev M.V.1, Savelyev I.V.2, Zyryanova N.V.3

1. Ph.D., Vaccine Research Institute, San Diego, CA, USA
2. Crimea Federal University, Simferopol, graduate student. [email protected]. Ph.D., independent researcher, Moscow.

It is known that DNA is a charge conductor and that in strong magnetic fields, ring currents are induced in aromatic ring structures of DNA bases. Also, some researchers have hypothesized that ring currents in DNA bases may be induced naturally in natural conditions and play a role in resonance functions of DNA in biological systems. Since for specific information transduction, resonances in DNA structures should depend on DNA sequence, we proposed models of ring currents in groups of bases and demonstrated that in these models, EM oscillations are induced that include groups of bases and depend on DNA sequence. We have also demonstrated experimentally that treatment of cell culture with weak millimeter waves changes DNA structure protecting it from UV damage which effect is in agreement with the hypothesis that week EM waves of certain frequencies are capable of increasing orderliness of DNA structure via resonance interactions.

INVESTIGATION OF THE EFFECT OF TERAHERTZ RADIATION ON CHANGES IN THE STRENGTH OF BONDING IN THE COMPLEX OF ALBUMIN WITH NITROGEN OXIDE

Nemova E.F.1,2, Cherkasova O.P. 1, Dultseva G.G.2

1Institute of Laser Physics, SB RAS
2Institute of Chemical Kinetics and Combustion, SB RAS. [email protected]

NO interaction with the THz-irradiated BSA preparations results in stronger local acidification due to NO NO2 conversion, which means weaker binding in NO-BSA complex. Thus, the complex formed by NO with BSA is somewhat weaker (and oxidation proceeds to a higher extent) for irradiated BSA than for non-irradiated BSA. NO oxidation proceeded with a higher rate and to a higher extent in the solutions with irradiated BSA than in the solutions with non-irradiated BSA. Possible candidate responsible for the observed changes may be proline fragment undergoing conformational changes induced by the terahertz radiation.

THE ROLE OF OXYGEN AND ITS ACTIVE METABOLITES IN BIOLOGICAL EFFECTS OF WEAK MAGNETIC FIELDS

Novikov V.V., Yablokova E.V., Fesenko E.E.

Institute of Cell Biophysics, Russian Academy of Sciences,
Institutskaya St., 3, Pushchino, Moscow Region, 142290, Russia E-mail: [email protected]

A preliminary mild partial degassing of a neutrophil suspension at an atmospheric gas pressure of 640 mm Hg was accompanied by a decrease in oxygen to 412 ng-atom O/mL and was shown to cause a significant (fourfold) decrease in neutrophil priming index on exposure to combined weak magnetic fields but did not affect the cell potential to generate a respiratory burst in response to an activator in the control.

TEMPERATURE DEPENDENCE OF THE WATER OH-STRETCHING RAMAN BAND

Pershin S.M., Pishchalnikov R.Y.

Prokhorov General Physics Institute, Russian Academy of Sciences, 119991, Moscow, Vavilovst., 38,[email protected], [email protected]

A linear temperature dependence of the water OH-stretching band (3200-3600 cm-1) in the nonresonant Raman experiments upon picoand femtosecond excitation has been observed [1]. The estimated linear coefficient is about 0.87 cm-1C-1. To model the Raman optical response in addition to the features of the excited states of a single water molecule, it is necessary to take into account the dynamics of the intermolecular interaction, as well as the fact that the water molecules are grouped into clusters of different sizes, whose contribution to the overall optical response in Raman scattering experiments can be significant. We have analyzed the calculated spectra of vibrational resonances for clusters of different sizes andmoleculararrangement. For each cluster, the temperature dependence was calculated. The resulting Raman spectrum was calculated as the sum of the contributions from each cluster with the corresponding weight coefficients. An optimal ratio of the contributions of different types of clusters was found, in which the temperature dependence is described most accurately.

NON-LINEAR EFFECTS IN SOLUTIONS WITH SMALL CONCENTRATIONS OF LUCIGENIN IN DEIONIZED WATER ACCORDING TO THE DATA OF OPTICAL MEASUREMENTS

Revina A.A., Tytik D.L., Busev S.A., Vysotskii V.V., Souvorova O.V., Kuzmin V.I.1, Gadzaov A.F.1

Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, Russia1Moscow Technological University (MIREA), Moscow, Russia

The luminescence spectra analysis of lucigenin solutions in deionized water depending on the concentration,which ranging from laboratory practice concentration (≈ 44 mg/l) to very high dilutions were obtained. That showed the presence of nonlinear effects for highly diluted solutions.

The obtained data on the dynamic light scattering from lucigenin solutions suggest that the solutions are not true and represent a lucigenin aggregates dispersion.

The special role of atmospheric gases dissolved in water in the form of bubbles is revealed. The sizes of bubbles lie in the same range as the sizes of lucigenin aggregates

FEATURES OF FIELD EMISSION OF ELECTRONS THROUGH STRUCTURED WATER SOLUTIONS OF SURFACE-ACTIVE SUBSTANCES AFTER THEIR MAGNETIC PULSE TREATMENT

Stepovich M.A., Shipko M.N.1, Sibirev A.L.1, Maslennikova O.M.2, Usoltseva N.V.3

Tsiolkovsky Kaluga State University, Russia, [email protected]
Lenin Ivanovo State Power Engineering University, Russia, [email protected]
2Central State Medical Academy of the Department of Presidential Affairs, Russia, [email protected] State University, Nanomaterials Research Institute, Russia, [email protected]

The effect of pulsed magnetic fields on the positional order of micelles in aqueous solutions of CTAB in the concentration range 0.14-54.88 mM was investigated. The method of gas-discharge visualization, based on the manifestation of the features of the field emission of electrons through drops of a solution, was used for the study. It is established that the picture of the angular distribution of the emitted electrons depends on the concentration of CTAB in the solution and the number of pulses of the magnetic treatment. It is shown that these pictures reproduce the stereographic projections of the planes of close packing of spherical micelles along which electrons can move, which makes it possible to study the features of positional ordering of molecular associates.

PRODUCTION OF REACTIVE OXYGEN SPECIES AND TUMOR NECROSIS FACTOR-ALPHA BY NEUTROPHILS AT ACTION OF LIGANDS OF TOLL-LIKE RECEPTORS

Teselkin Yu.О., Khoreva М.V., Babenkova I.V.

Pirogov Russian National Research Medical University, ul. Ostrovityanova 1, Moscow, E-mail: [email protected]

This paper presents a study on TLRs-mediated production of reactive oxygen species (ROS) and tumor necrosis factor- alpha (TNFα) by human peripheral blood neutrophils stimulated with zymosan (TLR2/6 ligand), peptidoglycan (PG, TLR2/1 ligand) and lipopolysaccharide (LPS, TLR4 ligand). Luminol- and lucigenin-dependent chemiluminescence (Lum-CL and Luc-CL) was used to detect ROS production. The concentration of TNFα was measured by enzyme immunoassay. The plots of dependences of the sum of light emission of Lum-CL and Luc-CL on concentration of each ligand were viewed as saturation curves. Comparison of the sum of light emission of Luc-CL (production of superoxide anion radical) and the sum of light emission of Lum-CL (total production of ROS) showed that the contribution of NADPH oxidase to the total TLRs-mediated production of oxidants can reach 40–50%. To compare the ability of ligands of TLRs stimulate the production of ROS and TNFα by neutrophils, stimulation indices were calculated. It has been established that activation of neutrophils with zymosan leads to higher production of ROS (8-fold higher) rather than TNFα production. Unlike zymosan, LPS stimulated in a greater degree (2-fold higher) the production of TNFαthan ROS production. PG is intermediate in position between these ligands. Thus, the production of effector molecules (ROS and TNFα) by human peripheral blood neutrophils depends on the nature of the TLR ligand.

INFORMATONAL ACTION OF ENVIROMENT ON THE REACTION OF THE BIOLOGICAL OBJECTS ON COMBINED MAGNETIC FIELD

Sheykina N.V., Bogatina N.I1

B. Verkin Institute for Low Temperature Physics &Engineering of National Academy of Sciences of Ukraine, Kharkov, Ukraine. E-mail: [email protected]
1 –National University of Pharmacy, Kharkov, Ukraine, E-mail: [email protected]

The analysis of the environment action on the dipole moment and magnetic moments of particles was fulfilled. It was shown that the electric and magnetic fields of electric and magnetic dipoles might be distorted essentially by the external objects. The permittiviityand permeability of the environment had to be taken into account. So the force of pushing away or attraction of particles, that had dipole or magnetic moment, to the boundary of two media might be changed when the external medium underwent from one phase to the other phase.

The new mechanism of starch grains (amyloplasts) moving is proposed. It is based on the distortion of the initial electric and magnetic fields of particles (ions and amyloplasts) by the external fields (electric and magnetic). The distortion caused the moving of amyloplasts to the center of cell and then to the upper part of the cell. The angle distributions of roots divergence from the horizontal plane confirmed it, so as the data of light and confocal microscopy. All effects observed in combined magnetic fields at cyclotron frequency of calcium ions may be explained by this mathematical model. The role of magnetic noise in excitement of the phase transition in water is discussed.

EFFECT OF LOW-INTENSITY DECIMETER ELECTROMAGNETIC RADIATION ON IRON INDICATORS IN RAT SERUM

Abbasova M.T., Hajiyev A.M.

Institute of Physiology named after academician Abdulla Karayev of Azerbaijan National Academy of Sciences, Baku,

[email protected]

Intensive development of radio and electronic communications leads to a significant “electromagnetic pollution” of the environment. Consequently, there is an interest in studying the effect on both biological environment and on human of low-intensity electromagnetic radiation (EMR), in particular, in the range of microwaves covering a range from 300 to 3000 MHz. Information about the level of iron in the serum and other parameters of iron is very important to assess the state of the body. The aim of our work was to study the serum iron in rats chronically exposed to low-intensity decimeter range EMR. Animals weighing 250-300 g were irradiated with decimeter radiation (frequency 460 MHz) on the device “Volna-2” (produced in Russia) in a metal cylindrical chamber, daily for 20 min to 28 days at a direct power density of 10 mW/cm2. The results showed that all three parameters of iron (serum iron, total iron binding capacity and free iron binding capacity of serum) in irradiated rats were significantly increased in relation to control animals (p<0.05).

The data suggest that decimeter range radiation can cause a redox shift in blood due to iron ions. The results will contribute to studies examining the effects of EMR on hematological parameters.

INFLUENCE OF TECHNO AND BIOSPHERE
ON THE LEVEL AND VARIATIONS OF GEOPHYSICAL VALUES

Dyukarev E.A., Zenchenko T.A., Lanskaya O.G., Nagorskiy P.M., Pustovalov K.N., Yakovlev G.A., Cherepnev M.S., Yakovleva V.S.E-mail: [email protected]

The article presents and discusses the results of parallel monitoring of geophysical quantities inside and outside the building. Purpose: to assess the influence of a building and the presence (absence) of a person in it on the change in the level and composition of variations in geophysical quantities.

The measured geophysical quantities were divided into three main groups based on the analysis of time and spectral characteristics of the variable components of geophysical quantities registered in different seasons of the year outside and inside the room.

AN INDIVIDUAL RESPONSE OF THE HUMAN CARDIOVASCULAR SYSTEM TO THE ENVIRONMENTAL STRESS

PishchalnikovR.Yu.1, VasinA.L.2, SarimovR.M.1, SasonkoM.L.2, GurfinkelYu.I.2

Prokhorov General Physics Institute, Russian Academy of Sciences, 119991, Moscow, Vavilovst., 38,1Instituteof Biomedical Problems, Russian Academy of Sciences, 123007, Moscow, Khoroshovskoye, 76A2Research Clinical Center of JSC “Russian Railways”, Moscow, Russia,Chasovayast. 203Space Research Institute of Russian Academy of Science, Moscow, 117997, Profsoyuznayast. 84/32 E-mail: [email protected]; Fax: +7-499-135-02-70

Three modes of external magnetic field (MF) generation have been employed to study an effect of the environmental stress on human cardiovascular system. The exposure setup, which is based on a Helmholtz-like MF generation system, has been created and used in this study [1-3].The eight subjects were participated in the 22-hour sessions of MF exposure. The weather conditions, the capillary blood velocity and blood pressure were also measured throughout the study. The high frequency part of the RR intervals power spectrum was used to estimate an influence of geomagnetic storm on cardiovascular system. The individual characteristics of cardiovascular response were detected for several subjects.

EVALUATION OF THE EFFECT OF LOW INTENSITY MILLIMETER WAVES IN COMBINATION WITH CADMIUM IONS AT THE WHEAT SEEDLINGS

Poghosyan G. H., Mukhaelyan Zh. H., Vardevanyan P.O.

Department of Biophysics, Faculty of Biology, Yerevan State University, 1 Alex Manoogian St., Yerevan, 0025, Armenia [email protected]

An experiment was conducted to examine the effect of isolated and combined action of different concentrations of CdCl2(50μM, 100μM) and EHF EMI on the growth, malondialdehyde (MDA) rate of wheat seedlings. A slight increase was recorded in the leaf /root (leaf > root) length and plant fresh weight in EMI- exposed group, while the levels of MDA in leaf were not changed significantly. High Cd concentration inhibited the seedlings growth and an increase of MDA content was observed. Research findings also showed that treatment of 50 μM Cd-exposed seedlings by MM-range EMI relatively improves the average plant parameters depending on EMI treatment duration, although registered indexes did not reach control values for intact seedlings.

EXTREMELY HIGH-FREQUENCY BAND INTERFEROMETER FOR NONCONTACT MONITORING OF BIOLOGICAL RHYTHMS FOLLOWED BY BODY SURFACE MOVEMENTS

Potekhina Yu.P., Kanakov V.A., Ikonnikov V.N., Mokhov D.E.1

AFS 52 LLC, Russia, [email protected], [email protected], [email protected] North-Western State Medical University named after I.I. Mechnikov, [email protected]

The on-line noncontact monitoring and display of human biologic rhythms are important for development of methods of functional diagnostics, chronomedicine, systems with biologic feed-back.

Interferometer is an instrument designed to measure a change of the distance from its antenna to the surface that reflects the electromagnetic waves by means of measuring of the differences of phases of electromagnetic wave emitted by the instrument and reflected be the object. The Company AFS 52 LLC, www.afs52.ru, Nizhny Novgorod (Russia) developed and manufactured a prototype of the 3 millimeter wave length range for biomedical research. This instrument allows to continuously and noncontact monitor human body movements (also through the clothes, chair seat and back, sofa) within a distance of 1 meter with a few microns tolerance. The flux density emitted by the instrument does not exceed 25 μW/cm2, thus, in line with sanitary norms, does not limit the exposure time. Figure 1 demonstrates the instrument in operation; example of recording of the human body relocations is shown in Figure 2. Both oscillogram and the signal spectrum clearly single out components linked with the respiratory and cardiac rhythms.

Fig. 1. Application of EHFB interferometer for Fig. 2. Example of human body surface movements, registration of respiratory and cardiac rhythms the oscilogram is above, the spectrum is bellow.

The extremely high-frequency band (EHFB) interferometer was tested on a group of 25 volunteers of both sexes and various ages. As result of measurements of cardiac contraction periods by means of the EHFB interferometer and portable electric cardiographs EK1Т-1/3-07 “AKSION” a good matching of results was established in 98% cases with a tolerance less than 1 millisecond. This way, the newly engineered instrument and relevant application technique allow to continuously and noncontactly monitor the respiratory and cardiac rhythms and analyze the parameter variability.

High sensitivity of the instrument for small relocations (within 0.01 mm) allowed to perform an experiment for detection and registration of cranial rhythm of 20 volunteers. During the experiments the volunteers were lying on back on the massage table. The location of the occipital and temporal bones was performed from under the massage table’s window and sideways. On the spectra of signals registered during more than one minute were identified components with a frequency from 4 through 8 cycles per minute that differed from the respiration rate. The respiration rate was imposed onto the experimental subjects by means of a demonstration of an image pulsating with a frequency of 15 cycles per minute. [The contactless measurement technique of biologic rhythms followed by human body surface movements – Patent RU 2651900, priority dated 14.02.2017]. The recorded cranial rhythm spectra consisted of one, and – more often – two multiple frequencies. For each experimental subject the main frequency of the cranial rhythm was individual, i.e. its value was replicated during the repeated measurement with a tolerance of 1/Тmes , where Тmes is the signal registration time. The present measurement accuracy of the cranial rhythm parameters is not sufficient to analyze its variability.

The obtained results substantiate applicability of EHFB interferometer for a remote contactless registration and monitoring of human cardiac and respiratory rhythms both during a short term (from 30 seconds – instant diagnosis: detection of critical-state symptoms), and extended time intervals (continuous monitoring, control over psycho-emotional state and fatigue). It is necessary to continue the cranial rhythm registration technique featuring an enhanced accuracy of evaluation of its time-related parameters.

VARIOUS PARAMETERS OF SPACE WEATHER AND THE CONDITION OF THE CARDIOVASCULAR SYSTEM OF HEALTHY AND SICK INDIVIDUALS

Samsonov S. N., Parshina S. S.1, Petrova P. G.2, Strekalovskaya A. A.2, Kobyakova S. E.

Institute of cosmophysical research and aeronomy, Yu. G. Shafer SB RAS, Russia, Yakutsk, [email protected];1-Saratov State Medical University n.a. V. I. Razumovsky, Russia,Saratov, [email protected] Institute of NEFU n.a. M. K. Ammosov, Russia, Yakutsk, [email protected]

The dynamics of the patients ‘ reversibility for hypertensive crises, myocardial infarction and stroke to emergency medical care in Yakutsk city depending on the daily dynamics of electromagnetic radiation of the Sun and geomagnetic disturbance is analyzed. The reaction of the cardiovascular system of healthy individuals was studied in a multi-latitude experiment (subarctic zone – Yakutsk, middle latitudes – Saratov) according to the daily dynamics of the t-wave symmetry of the electrocardiogram (KST ECG). The relationship between the emergence of hypertensive crises with the indicators of both electromagnetic radiation of the Sun and geomagnetic disturbance (2 peaks) was found, 3 peaks of reversibility were revealed for patients with myocardial infarction and stroke. The response of myocardium of healthy individuals at different latitudes was determined, first of all, to the change of geomagnetic disturbance.

THE INFLUENCE OF WEAK ELECTRIC FIELDS ON THE GROWTH OF LLC TUMORS (ADENOCARCINOMA OF THE LUNG LEWIS) OF LABORATORE MICES

E.P.Teterin, C.A.Anisimova, S.V.Zinoviev1, A.N.Evdokimov

Kovrov State Technological Academy by V.A.Degtyarev, E-mail: [email protected]
1Research Institute of experimental diagnosis and therapy of tumors of the Russian cancer research center by N.Blokhin An increase in the growth of the tumor of LLC is shown depending on the characteristic frequency and duration of irradiation by the electric field.

MICROBIAL EFFECTS OF ELECTROMAGNETIC RADIATION OF EXTREMELY HIGH FREQUENCY: NEW FINDINGS, MECHANISMS AND SIGNIFICANCE

Trchounian A.*

Yerevan State University, Yerevan, Armenia; *[email protected]

Electromagnetic field of extremely high frequencies at low-intensity is a new environmental factor the level of which is increased, as technology advance. This field is irradiated by satellite telecommunication, radiometry, traffic and military radars and/or remote sensing devices. This field is of interest due to findings that (1) bacteria and other cells might communicate each with other by electromagnetic field of sub-extremely high frequency range, and (2)different microbial effects of electromagnetic field of extremely high frequencies have been revealed [1].

The electromagnetic field of 51.8 or 53.0 GHz affected Escherichia coli and many other bacteria or microalgae mainly depressing their growth changing growth properties and viability and disturbing membrane-associated transport and enzymatic activity. These effects were non-thermal and depended on different factors. The effects of electromagnetic field have some similarities and differences with a number of bacteria studied. Distinguishing effects of morphology have been documented with Gram-positive and Gram-negative bacteria. The significant cellular targets for electromagnetic field of extremely high frequency effects could be water, cell plasma membrane and genome. The changes in water features by their turn can alter membrane proteins conformation, their hydration degree and other properties resulting in their altered activity. All this can even change cells morphology and sizes Actually, scanning electron microscopy analysis showed that almost all cells in the population irradiated by 53 GHz have spherical shapes similar to non-irradiated ones, but they have 1.3-fold increased diameters in case of irradiated cells [2]. This field has also affected hydrogen production by bacteria [3].

The model for the of electromagnetic field of extremely high frequencies interaction with bacteria is suggested; an essential role of the membrane-associated proton FOF1-ATPase, key enzyme of bioenergetic relevance, is proposed. Interestingly, a role of ATPase might be common for different cells: increased synthesis of ATP in biological effects of electromagnetic field is also offered for human cells [4] and for the other type of electromagnetic radiation.

The consequences of electromagnetic field of extremely high frequencies interaction with bacteria are the changes in their sensitivity to different biologically active chemicals, including a number of antibiotics. Importantly, the combined action of electromagnetic field of extremely high frequencies and antibiotics resulted with more strong effects.

These effects are of significance for understanding changed metabolic pathways and distinguish role of bacteria in environment, they might be leading to antibiotic resistance in bacteria. The effects might have applications in the nature protection, development of technique, therapeutic practices and food protection technology.

ELECTROMAGNETIC RADIATION OF MOBILE TELEPHONES: YOUNGER GENERATION IN THE GROUP OF ESPECIAL RISK

Khorseva N.I.1, 2, Grigoriev Yu.G.3, Grigoriev P.E4,5

PHYSICO-CHEMICAL INFLUENCE ON MEDICINAL PLANTS

Svetlana S., Shutava H., Mazets Zh.1
Central Botanical Garden, NAS of Belarus
1Belarusian State Pedagogical University named M. Tank
220012, Belarus, Мinsk, st. Surganova 2 v, e-mail: [email protected]

This research is devoted to investigation effects of presowing treatment by electromagnetic radiation of the millimeter- wave range (EMR) and exogenous aminolevulinic acid (ALA) in ultra-low concentrations in medicinal plants Calendula officinalis L. and Nigella sativa L.. Microwave EMR in two frequency bands, 53.57-78.33 GHz (wide range mode) and 64.00-66.00 GHz (narrow range mode), has been selected to study the physical effects on seeds, with a treatment exposure time of 20, 12, or 8 min and ALA in micro- and nanoconcentrations have been used: 1) 10-6; 2) 10-7; 3) 10-9; 4) 10-11. The experiments were conducted under laboratory and field conditions. Morphometric and biochemical parameters of plants at different stages of ontogenesis were studied. Original data about EMR and ALA influence on the content of phytohormones in seedlings and the qualitative composition and quantitative content of saturated and unsaturated fatty acids, as well as individual biologically active compounds in the seeds of the plants C. officinalis and N. sativa under study were obtained.

EXPERIENCE AND PERSPECTIVES OF OBJECTIVE EVALUATION OF SYMPTOMS DISORDERS OF POSTURE WITH THE USE OF MOBILE TECHNOLOGY

Arsenev A.V. 1, Vasilevich S.V. 1, Dudin M.G. 1, Sukhov T.M. 2 1Children’s Rehabilitation Center of Orthpedics and Traumatology “Ogonek”2Baltic State Technical University “Voenmeh” named after D.F. Ustinov St. Petersburg, Russia
[email protected]

Clinical evaluation of orthopedic status patients with deforming dorsopathies is the subjective opinion expert on qualitative and quantitative manifestation of certain symptoms. Examination must be confirmed by objective diagnostic methods to comply with the principles of evidence-based medicine. The purpose of this work is the definition of the diagnostic importance of the new objective method for diagnosis of posture and deformities of the spine. The technology, based on the processing of digital images of the human body by specialized computer program was invented. A digital image of the patient may be obtained using a wide range of mobile devices. The method attracts by it availability, absolute safety for the patient and a high reliability of diagnostic results. This diagnostics allows to complete the clinical examination of the patient by objective documented visualization. In addition, the technology can be used as diagnostic telemedicine.

EFFECTS OF WEAK EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELD ON HUMAN SLEEP

Blokhin I.S. Arsenyev G.N.1, Dorokhov V.B.1

P.N.Lebedev Physical Institute of the Russian Academy of Sciences. Moscow
1-Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences. Moscow. E-mail: [email protected]

Influence weak (less than 0,2 мкТл) a pulse electromagnetic field of extremely low frequency (2, 4, 8, 16, 20, 32, 40 Hz) is shown on various characteristics of a human sleep by means of sleep questionnaires. Field exposition duration – it is continuous during the night. In experiments the device – “Smart Sleep”, author’s development – the certificate of conformity of GOST P 0159555 was used of 15.12.2017.

THE INVESTIGATION OF DRUG PHARMACOLOGICAL ACTIVITIES USING THE METHOD OF OPTICAL PECTROSCOPY

Gorbunov A.M., Ilyenko V.A.1, Vladimirova E.S.2

A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, RAS,Leninsky Prospeсt, 31, Moscow, 119071, Russia, e-mail: [email protected] Doctor Clinic, Moscow, Russia
2N.V.Sklifosovsky Research Institute for Emergency Medicine,
3 Bolshaya Sukharevskaya Sq., Moscow 129090, Russia

The search for new medicines is carried out by joint efforts of pharmacology, chemistry, pharmacy. The pharmacological activity of various substances is studied empirically. The present study has been based on the “trial and error” method where pharmacologists test the existing substances for their belonging to a particular pharmacological group, by using a set of pharmacological techniques. It should be noted that this way of searching for and creating new drugs is very laborious: on average, per one worthy drug there are 5-10 thousand compounds studied.

The method we have proposed for determining the pharmacological activity does not require a large amount of substance or high costs.

The pharmacological activity of aqueous and aqueous-alcoholic solutions of substances was evaluated from the change in the structure of water in the infrared region [1-3].

We have shown that if the drug exhibits an immune-stimulating and anti-inflammatory effect, then an absorption band appears in the IR spectrum in the region of 1225-1215 cm-1, and in the region of 1215-1208 cm-1 if the drug produces an antiviral, bactericidal effect. The absorption bands appearing in the region of 1208-1200 cm-1 indicate the presence of the pharmacological effect similar to that of hormones [1-3]. It is known that to compensate for the negative effects of physical, chemical, biological, and psychogenic factors, an important aspect is the adaptive restructuring of the body to enhance its protective properties. In order to survive, the body begins producing adrenaline; the thermoregulatory system starts up (35-420), and the brain generates electromagnetic fluctuations (1-30 Hz). The figure shows that the concentration of the adrenaline release in the body (10-1, 10-2, 10-3, 10-4 % for curves 2, 3, 4, 5, respectively) depends on the physiological state occurring in response to a variety of extreme impacts. Also, there are shown the spectra of a 5% glucose solution (curve 6), a 0.9% NaCl solution (saline) (curve 7), Ringer solution (curve 8), and of the drugs used in practical medicine for emergency care to launch important processes and accelerate metabolism. The increase in the intensity of IR absorption bands for drugs in relation to the absorption bands for the normal body condition at a temperature of 36.50indicates the pharmacological effect of enhancing the body’s defense systems.

References

1. Gorbunov A.M., Ilyenko V.A., Vladimirova E.S. Pharmacological effect of ethanol aqueous solutions. //XX Mendeleev Congress on General and Applied Chemistry, Ekaterinburg: September 26-30. Ural Branch of the Russian Academy of Sciences, 2016. V.4, p. 476.
2. Gorbunov А.М., Vladimirova E.S., Ilyenko V.A. Pharmacological effect of aqueous solutions of drugs and watermodified by electromagnetic field.// VII International Congress “The weak and super-weak fields and radiation in biology and medicine”, 2015, St. Petersburg, A. Symposium, 31.

3. Gorbunov А.М., Ilyenko V.A., Bocharova O.A. The structure and value of bound water in phytomix-40 preparation.// Russian Journal of Biotherapeutic, 2008, V. 7, 1, 39.

USAGE OF DIFFERENTIATED METHODS OF ACUPUNCTURE FOR TREATMENT OF PAIN SYNDROME OF VERTEBROGENIC GENESIS IN CHILDREN AND ADOLESCENTS

Zaltsman P.L.

Children’s Rehabilitation Center of Orthopaedics and Traumatology «Ogonyok» Saint-Petersburg, Russia [email protected]+7(921)-631-80-70 8(812)-421-42-36

This research aims at analyzing the effects of differential reflexоtherapy technique on the pain syndrome of lumbosacral localization of vertebrogenic genesis of children and adolescents.

One hundred twenty-seven patients aged from 8 to 18 years were observed and treated. All patients were divided into 2 groups – the main (72 children) and control (40 children). Patients of the main group were treated using the differentiated reflexotherapy technique apart from generally accepted treatment (remedial gymnastics, massage, physiotherapy, medication). Acupuncture was not provided for patients of the control group.

Comparative analysis of the treatment results demonstrates that the most effective treatment proved to be exploiting the differentiated reflexotherapy technique which made the pain syndrome reduced in shorter terms. The pain syndrome in the main group disappeared at 3rd-27th day from the beginning of the treatment while in the control group it happened only at 23rd-55th day from the beginning of treatment.

HOW TO LIVE IN AN ERA OF GLOBAL MICRUWAVE “SMOG”

Ovsyannikov V.A.

Ioffe A.F. Physico-Technical Institute, Russia E. email: [email protected]

That radiation frequencies are harmful to human health, scientists say about that a long time, but without tangible results. Why this happens with harmful factor for human health? Because people do not feel at this harm, and that positive effects from the use of radio communication, radar and radio equipment is so entrenched in our lives that without them life in a modern society, it is difficult to imagine. We list only the undisputed harm to human health. Scientists of the Military medical Academy in St. Petersburg and the Institute of Biophysics in Pushino showed that microwave radiation even a small intensity during long exposure suppresses immune and nonspecific protective human capacity on a big statistical material. Without immunity the human body is defenseless before the pathogenic effects, and its genetics vulnerable to any external shocks. It is the most clearly manifested in the embryonic stage of development. At the 10-12 week embryo maturation, when just starting to form its protective systems, microwave radiation suppresses they, and embryo died of any infections or genetic changes. So now die 20% -30% of embryos in the developed world, including Russia. Doctors call this process “miscarriage”, in reality, it’s killing babies by means of radio transmitters near inhabited or worked, walked these women. The embryo does not legally considered a person, and the only reason the true killers has not attracted the due responsibility.

THE APPLICATION OF MULTICHANNEL SQUID-SYSTEMS FOR THE ASSESSMENT OF THE BRAIN MAGNETIC ACTIVITY LOCALIZATION IN THE PROCESS OF THE “FATIGUE/STRESS” STATUS CORRECTION LOW-FREQUENCY MAGNETIC FIELD.

Sinelnikova I.A., Lobkayeva E.P.

RFNC-VNIIEF, Russia, 607188, Sarov, Nizhny Novgorod region, Mira Ave, 37 e-mail: [email protected]

This paper presents the investigation results of modeling «fatigue/stress» and the effect of low-intensity alternating magnetic field (MF) on the characteristics of the human magnetoencephalogram (MEG). Maximum value of magnetic induction in the center of the working area of 1.4 MT and 1.2 MT, the form of the signal – a damped sinusoid, frequency ~100 Hz, the repetition rate of the signal 1.0±0.1 Hz.

Neurophysiological changes caused by the action of MF indicate a decrease in the activation of brain structures and emotional and cognitive excitation and tension, increase in overall performance.

A large reactivity of the right hemisphere structures to the load and the impact of low-frequency magnetic field was revealed. This indicates a change in the ability to summarize information.
Литература
1. Синельникова И.А., Лобкаева Е.П. Технология исследования и оценки функционального состояния центральной нервной системы при воздействии различных факторов с использованием СКВИД-магнитоэнцефалографии // Сборник докладов IV Международной конференции. – Саров: 2014., С. 323-338. (www.biophys.ru/archive/sarov2013/proc-p77.pdf)

2. Патент РФ No 2290970 МПК6 A61N 2/02. Устройство для воздействия магнитным полем на биообъект / Лобкаева Е.П., Кудряшов Л.В., Комиссаров В.И., Девяткова Н.С., Шевцов И.Д. // Изобретения. 2007. No 1.
3. Патент РФ No 2432972 МПК6 A61N 2/00. Способ коррекции функционального состояния организма / Синельникова И.А., Лобкаева Е.П., Девяткова Н.С., Шевцов И.Д., и др. // Изобретения. 2011. No 31.

BIOREREGULATOR, OBTAINED FROM CATTLE BLOOD SERUM, PROVIDES WOUND-HEALING EFFECT

Chekova V.M., Ilyina A.P., Shumilina D.R., Yamskova V. P.1, Yamskov I.A.

Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russia1Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

In various tissues of vertebrate animals, including, and in blood serum, protein-peptides complexes which in low doses exerted impact on the main biological processes have been found and also stimulated recovery and a reparation in the injured and pathologically changed tissues. The biological effect of protein-peptides complexes is characterized by existence of tissues, but not specific specificity. They have been united in new group of biologically active substance – the membranotropny homeostatic tissues-specific bioregulators (MHTB), on the basis of similarity of their physical and chemical properties and the nature of biological effect. In this work studied MHTB isolated from cattle blood serum. Basis of MHTB is the protein-peptides complex containing peptides with a molecular masses 1151±2 And 1447±2 and protein-modulator with a molecular weight of 66439±2 with the partial N-terminal amino-acid sequence of DTHKSEIAHRFKDLGE-, a homologous nature molecule of bull serum albumine. By method of trypsin hydrolysis, it has been established that this protein-modulator is one of isoforms of serum albumineB. taurusat number gi│1351907in the Uniprot database. It is important to note that this protein-peptides complex shows chaperone-like activity in 75- 200 nM which the intermolecular aggregation of BSA and lysozyme induced dithiotreitol was completely blocked. On model of a skin wound at mouse of in vivo it has been shown that the studied MHTB stimulated processes of recovery and a reparation in the field of an experimental wound which developed on the mechanism of epimorphic regeneration in low doses – hair follicles, subcutaneous fat were formed, actively there was an epithelization of area of skin defect and formation of cicatricial fabric.

MILLIMETER WAVES OF NON-THERMAL INTENSITY INDUCE THE RESISTANCE OF PLANTS TO PATHOGENS AND PESTS

VayshlyaO.B., LikhomanovaE.D., KozhemyakinA.M.1

NationalResearchTomskStateUniversity, Tomsk, 634050, Russia1LLC «Spinor», Tomsk, 634009, Russia
E-mail: [email protected]

Important issue due to practical using of millimeter-waves range of electromagnetic waves is considered. Experiments were conducted to study the effect of low intensity extremely high frequency electromagnetic radiation (EHF EMR). Itrecorded from specific organisms, induce the resistance of plants to harmful insects and Haplothrips tritici, mold pathogenic fungi from the genus Alternaria, Helminthosporium andFusarium. Obtained results have been shown a reduction in the incidence of fungal diseases in spring wheat seeds and

the possibility of controlling the number of pest populations.

Литература

1. Betskii O.V., Devyatkov N.D., Kislov V.V. // Crit. Rev. Biomed. Eng., 2000, 28, 247-268
2. Вайшля О.Б., Лихоманова Е.Д. Новый аспект применения низкоинтенсивного излучения в защитеAbiessibirica от уссурийского полиграфа // Матер.Межд. конф. «Мониторинг и биологические методы контроля вредителей и патогенов древесных растений: от теории к практике», Москва, 2016, С. 48–49.

DISEASE LAW

(812) 777-04-27, 309-38-30, www.d-med.pro e-mail: [email protected]

Donchenko E. V.

Clinic of Environmental Medicine, Russia, St. Petersburg, ul. Kuibyshev, d. 22

In the medical literature, the idea of the disease is mainly focused on the influence of external factors on the development of the disease Analyzing the work of the body, in different States and faced with multiple reproduction of certain reactions in almost 100% of cases. I formulated these reactions in the form of the laws of the human body.

Disease law

The disease is an adaptive reaction of the body to the imbalance that has arisen due to various reasons. The body is a kind of self-adjusting system, which is constantly in a mobile balance with the environment. With the change of various geophysical parameters (magnetic field, temperature, day length, gravity, and more), the body reconstructs thesystem of functioning to the required optimal level.

If the body’s resources are in good condition, then even with the development of an imbalance, the body neutralizes the negative reactions, without creating for us painful manifestations. If the body has a deficit of certain substances; vitamins, micro -, macroelements, protein necessary to restore the disturbed balance, overload the endo and exotoxins, blocking the receptor apparatus of the cell, which prevents its normal metabolism, then it signals us about it in the form of painful conditions.

In the event of a painful condition in infectious diseases, and chronic in the development of conditionally can be identified 2 points; A – the beginning of the disease, B – the end of the disease, recovery. Between these points there is a set of reactions that the body must implement. If this does not interfere, then, reaching point B, restored the disturbed balance, and comes recovery.

So, the law of the disease consists of the following provisions

1. The disease is an adaptive reaction in response to the disturbed balance that occurs for various reasons.

2. The painful condition develops as a result of excess toxins, lack of necessary substances in the body,

3. The development of the disease state is affected by the weakening of the immunological protection.

4. To restore the disturbed health of the body it is necessary to give the opportunity to implement genetically programmed

reactions from the point A the beginning of the disease to the point b – recovery.

5. You can not thoughtlessly prescribe pharmaceutical products that block the natural path to recovery.

6. The body needs help to restore the disturbed balance. due to the release of accumulated toxins, on the one hand, and fill the deficit of necessary substances ( protein, vitamins, minerals, enzymes, etc.) on the other. Only in this case, a full recovery is possible.

7. Among the triggers in the occurrence of disease play a great role violated psihologicheskie the person.

For more than 25 years of practice, based on the total understanding, we have treated more than 30 000 people with different types of pathology with an efficiency of 80%-90%. Thus, a proper understanding of the laws of the disease will allow to develop more effective methods of treatment, and to achieve long-term resistant remission.

Литература

1. Донченко Е.В. Возможности использования клеточных биорегуляторов в эндоэкологической реабилитации больных с хроническими заболеваниями внутренних органов. // Ревитализация .Москва No11. 2016. С.40-47
2. Донченко Е. В. Средства и методы экологической медицины в лечении и профилактике хронических заболеваний внутренних органов. Реферат по выполненным научным работам на соискание ученой степени доктора медицинских наук. СПб. 2008. 31 с.

3. Донченко Е.В. Эндоэкологический подход к лечению хронических заболеваний. // IV Вестник Санкт-Петербургской государственной академии. И.И. Мечникова. рp. 186-188. 2003.
4. Донченко Е.В. Гармония души и тела. // Экономика и медицина. Москва. No 8. 2008. С28-5. 5. 5.Donchenko E.V., Shcherbakova I.V., Sleptsov R.V. // Effectiveness Of Ecological Human Medicine In The Treatment Of Diseases Of TheInternal Organs// Международный медицинский симпозиум «Euromedica-2010». 03.06.2010-04.06.2010. Hannover // http://www.congress-euromedica.de/abstrakt2010 /effectiveness-of-ecological-human-medicine-in-the-treatment-of- diseases-ofthe-internal-organs.html

6. Donchenko E. V. // Vascular Atherosclerosis and Possibilities Of Ecological Human Medicine In Its Treatment //Международный медицинский симпозиум «Euromedica-2010». 03.06.2010-04.06.2010. Hannover // http://www.congress-euromedica.de /abstrakt2010/vascular-atherosclerosis-and-possibilities-of-ecological-human- medicine-inits-treatment.html

Full text (mostly in Russian) here:

http://biophys.ru/archive/congress-2018.pdf