Low and Superlow Congress 7, 2015

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Low and Superlow Fields and Radiation in Medicine and Biology

International Congress since 1997

LSFRMB 2015

THEORETICAL PROBLEMS IN MAGNETOBIOLOGY

Binhi V.N.
General Physics Institute RAS; Moscow State University, Moscow, Russia; E-mail: [email protected]

A review of theoretical problems in magnetobiology is presented. Discussed are the general properties of magnetobiological effects and a few mechanisms that can explain these properties correctly. It is shown that the unified [common] basis of a correct mechanism is the dynamics of a magnetic moment in the magnetic field. A “theorem” is proven that the nonlinear spectral and amplitude characteristics of the primary magnetoreception follow the postulate that nonthermal biological effects exist, the law of physics, and a sole logically justified and general presumption. The properties of a mathematical model of the mechanism and the perspective of a good-enough solution to the problem are considered.

Литература

1. Binhi V.N. Theoretical concepts in magnetobiology // Electro- and Magnetobiology, 2001, V. 20, N. 1, P. 43-58. 2. Бинги В.Н., Миляев В.А., Чернавский Д.С., Рубин А.Б. Парадокс магнитобиологии: анализ и перспективы

решения // Биофизика, 2006, Т. 51, No 3, Стр. 553-559.

LONG-TERM FLUCTUATIONS OF ELECTRICAL CONDUCTIVITY OF DISTILLED WATER Ageev I.M., Rybin Yu.M., Shishkin G.G., Bubnova M.D.

NONVALENT ENZYME-SUBSTRATE AND ENZYME-INHIBITOR COMPLEXES OF PENICILLOPEPSIN

R.E. Aliev
Baku State University, Azerbaijan, AZ1148, Z. Khalilov, 23, E-mail: [email protected]

Литература

  1. Антонов В.К. //Химия протеолиза. М. Наука, 1991, с.504.
  2. Andreeva N. S., Rumsh L.D.// Protein Science, 2001, v.10, p.2439-2450.
  3. James M.N.G., Sielechi A.R.// J.Mol. Biol, 1983, v.163, p299-361.
  4. Алиев Р.Э.// Вестник Бакинского университета, 2014, No3, с.149-155.
  5. Попов Е.М.// Структурно-функциональная организация белков. М. Наука, 1992, с.358

STUDY UV-INDUCED CHANGES OF STRUCTURAL AND FUNCTIONAL PROPERTIES NATIVE FICIN AND FICIN IMMOBILIZED ON THE MATRIX OF ACID-SOLUBLE CHITOSAN
Artuhov V.G., Sazykina S.M., Koroleva V.A., Holyavka M.G.
Voronezh State University, 394006 Voronezh, Universitetskaya square 1,
e-mail: [email protected], [email protected]

The research on the development of highly heterogeneous preparations based on immobilized enzymes is gaining popularity and necessity. The attaching the enzyme to an insoluble carrier not only improves the stability, but also the effectiveness of the medicine by controlling the reaction process. For the immobilized enzyme inactivating number of possible mechanisms (such as UV radiation, temperature) is substantially less than in the case of soluble proteins.

For immobilized ficin photoprotective effect of acid-chitosan matrix had identified which may be due to the following reasons: 1) chitosan reacts with the enzyme molecule forming a more photoresistant complex than the native protein molecule; 2) chitosan probably binds and/or shields the active photoproducts of the free radical nature, preventing photooxidation of a certain number of ficin amino acids under UV irradiation.

Литература

  1. Артюхов В.Г. Биофизика / учебник для вузов – Москва: Академический Проект; Екатеринбург:Деловая книга, 2009. – 294 с.
  2. Березин И.В. Биотехнология: учеб.пособ. для вузов. В 8 кн / под ред. Н.С. Егорова, В.Д. Самуилова. кн7: Иммобилизованные ферменты / И.В.Березин, А.А. Клячко и др. Москва: Высшая Школа, 1987. 159 с.
  3. Владимиров Ю.А., Потапенко А.Я. Физико-химические основы фотобиологических процессов.Москва: Дрофа, 2006. – 285 с.
  4. Егорова Т.А. Основы биотехнологии – М: Издат. Центр «Академия», 2003. – 208 с
  5. Логинова О.О., Холявка М.Г., Артюхов В.Г. // Биофармацевтический журнал. – 2015. – No 2. – С. 13-16.
  6. Мосолов В.В. Протеолитические ферменты. – Москва: Наука, 1971. – 404 с.
  7. Рощупкин Д.И., Артюхов В.Г. Основы фотобиофизики: учеб. Пособие. – Воронеж: ВГУ, 1997. – 116 с.

ANTIRADICAL ACTIVITY OF WHEAT GERM OIL
Babenkova I.V., Buravlev E.A., Buravleva K.V.1, Teselkin Yu.O.Pirogov Russian National Research Medical University, E-mail: [email protected] Institute of Physico-Chemical Medicine, E-mail: [email protected]

Antiradical activity(ARA) of wheat germ oil has been studied by chemiluminescence system hemoglobin-H2O2- luminol. It was shown that ARA of rabbit plasma increases by 2-3 times after a single oral administration to rabbits wheat germ oil. Antiradical properties of wheat germ oil may be one of the important biological mechanism of the human organism.

Литература

  1. Da Costa L.A., Badawi A., El Sohemy // Ann Nutr Metab, 2012, V. 60, Suppl. 3, P. 2736.
  2. Теселкин Ю.О., Бабеноква И.В., Любицкий О.Б., Клебанов Г.И., Владимиров Ю.А. // Вопр. мед. химии,1998, Т. 44, No1, С. 7076.

OSCILLATORY PROCESSES IN BIOLOGICAL SYSTEMS AND HIGHLY DILUTED AQUEOUS SOLUTION DUE TO THE PRESENCE OF REACTIVE OXYGEN SPECIES AND NITROGEN OXIDESL.V. Belovolova, M.V. Glushkov
Institution of the Russian Academy of Sciences Institute of General Physics,
119991, Russia, Moscow, ul. Vavilova 38, E-mail: : [email protected] .

Литература

  1. Бурлакова Е.Б. Конрадов А. А., Мальцева Е. Л. // Химическая физика. 2003, т. 22, No2, с.21
  2. Тимашев С.Ф.// Фликкер-шумовая спектроскопия. Информация в хаотических сигналах. М. Физматлит,2007

THEORETICAL PROBLEMS IN MAGNETOBIOLOGY

Binhi V.N.
General Physics Institute RAS; Moscow State University, Moscow, Russia; E-mail: [email protected]

A review of theoretical problems in magnetobiology is presented. Discussed are the general properties of magnetobiological effects and a few mechanisms that can explain these properties correctly. It is shown that the unified [common] basis of a correct mechanism is the dynamics of a magnetic moment in the magnetic field. A “theorem” is proven that the nonlinear spectral and amplitude characteristics of the primary magnetoreception follow the postulate that nonthermal biological effects exist, the law of physics, and a sole logically justified and general presumption. The properties of a mathematical model of the mechanism and the perspective of a good-enough solution to the problem are considered.

Литература

1. Binhi V.N. Theoretical concepts in magnetobiology // Electro- and Magnetobiology, 2001, V. 20, N. 1, P. 43-58. 2. Бинги В.Н., Миляев В.А., Чернавский Д.С., Рубин А.Б. Парадокс магнитобиологии: анализ и перспективы решения // Биофизика, 2006, Т. 51, No 3, Стр. 553-559.

EFFECT OF ALTERNATIVE ELECTRIC FIELDS OF DIFFERENT DIRECTIONS ON THE CRESS ROOTS GRAVITROPIC REACTION IN VERTICAL AND HORIZONTAL STATIC MAGNETIC FIELD

Bogatina N.I. , Sheykina N.V.1, Linnik A.S.

B. Verkin Institute for Low Temperature Physics &Engineering of National Academy of Sciences of Ukraine, Kharkov, Ukraine. E-mail: [email protected]
1 –National University of Pharmacy, Kharkov, Ukraine, E-mail: [email protected]

The gravitropic reaction of cress roots was investigated in static vertical magnetic field and alternative electric field of different directions. The alternative electric field was tuned to cyclotron frequency of Ca2+ ions in vertical magnetic field. Three variants were investigated. In the first one the magnetic field and the electric field are parallel between themselves and gravitational vector g. So the roots were located perpendicular to all of them. In second and third variants the magnetic field and the electric field are perpendicular to each other and roots were located either parallel to electric field (second variant) or perpendicular to it ( third variant). In the first and second variants the essential inhibition of gravitropic reaction was observed.

The analogous investigation has been fulfilled for horizontal magnetic field. The alternative electric field was tuned to cyclotron frequency of Ca2+ ions in horizontal magnetic field. Six different variants were investigated. In the first variant the alternative electric field was parallel to magnetic one and roots were perpendicular to both of them. In the 2- nd variant the electric field was perpendicular to magnetic one and parallel to g, the roots were perpendicular to all of them. In the 3-d variant the electric field was perpendicular to magnetic one and parallel to g, the roots were parallel to magnetic field. In the 4-th variant the electric field was parallel to magnetic one and roots were parallel to both of them. In the 5-th variant the electric field was perpendicular to both the magnetic field and g and the roots were parallel to magnetic field. In the 6-th variant the electric field was perpendicular to both the magnetic field and g and the roots were parallel to electric field. The essential inhibition of gravitropic reaction yas been observed only in the first variant. In the 6-th variant the essential decreasing of gravitropic reaction has been observed only at the beginning.

WATER COMPLEXES TAKE PART IN BIOLOGICAL EFFECT CREATED BY WEAK COMBINED MAGNETIC FIELD

Bogatina N.I. , Sheykina N.V.2, B. Verkin

Institute for Low Temperature Physics &Engineering of National Academy of Sciences of Ukraine, Kharkov, Ukraine. E-mail: [email protected]
1 –National University of Pharmacy, Kharkov, Ukraine, E-mail: [email protected]

It was revealed experimentally that at small level of magnetic field’s noise (less than 4μT/Hz0.5) the dependence of gravitropc reaction of cress roots on frequency had a fine structure/ The peak that corresponded to the cyclotron frequency of Са2+ ions for the static component of combined magnetic field that was equal to 40μT became split up into three peaks ( f1 = 31/3Hz, f2 = 32.5Hz и f3 = 34 Hz. (fig.1)

The frequency f1 corresponded to the Са2+ ion (theoretical value 31.6 Hz), the frequency f2 corresponded to the hydronium ion H3O+ (theoretical value 32.9 Hz), the frequency f3 corresponded to OH- ion (theoretical value 35 Hz).

Taking into account the influence of combined magnetic field on hydronium ions and Del Giudice’ hypothesis one may throw away doubts about the possibility of ion cyclotron resonance. The hydronium ions are unusual because they have a long free path length.

It was revealed that pH of the distillated water changed under the treatment in combined magnetic field tuned to cyclotron frequency of hydronium ion. Such changes in pH had to lead to the biological effects on the molecular ,cell and organism levels.

THE THRESHOLD OF CRESS ROOTS GRAVITROPIC REACTION SENSITIVITY FOR STATIC AND ALTERNATIVE MAGNETIC AND ELECTRIC FIELDS
Bogatina N.I. , Sheykina N.V.1

B. Verkin Institute for Low Temperature Physics &Engineering of National Academy of Sciences of Ukraine, Kharkov, Ukraine. E-mail: [email protected]

1 –National University of Pharmacy, Kharkov, Ukraine, E-mail: [email protected]

The dependence of cress roots gravitropic reaction on the inductance of the static magnetic field in the region of its values from 1μT until 500 μT ( the alternative magnetic field was absent) has been investigated. There was the threshold in the dependence at the inductance value 1 – 2 μT. The dependence of cress roots gravitropic reaction on the amplitude of alternative magnetic field for different frequencies ( the static magnetic field was absent) has been investigated/ It was shown that the dependence had the threshold character. The threshold had the value 1 – 2 μT. The gravitropic reaction dependence on amplitude did not depend on frequency and it was the same for Schuman’s frequencies as for any other ones.

The same experiments have been fulfilled for static and alternative electric fields in the regin of values 0 until 300 V/m. It was shown that neither static nor alternative electric field had influence on the gravitropic reaction while the magnetic field was absent

STATUS SIGNALING PATHWAY JAK/STAT/SOCS IN AGRANULOCYTIC WHOLE BLOOD OF PATIENTS WITH PNEUMONIA UNDER THE INFLUENCE OF LOW-INTENSITY MICROWAVE IRRADIATION
S.S.Bondar, V.I.Petrosyan, I.V.Terekhov
Tula State University, prospect Lenina 92, 300012 Tula, Russia. E-mail: [email protected]

In experiments on the model of intercellular cooperation cell culture whole blood the convalescence phase of community-acquired bacterial pneumonia were studied the effects of low intensity microwave radiation on the state of the JAK/STAT/SOCS signaling pathways in mononuclear cells. The study showed that the sensitivity of intracellular signaling systems to the effects of low intensity microwave radiation, which is manifested by increased intracellular concentrations of Janus kinases and SOCS proteins with a simultaneous decrease in the content of STAT factors.

Литература

Sinitsyn N.I., Yolkin V.A., Gulyaev Yu.V. et al. //Critical Reviews in Biomedical Engineering. 2000;.28(1-2);269.Петросян В.И. //Письма в ЖТФ, 2005, Т.31, Вып. 23, с. 29-33.
Бpилль Г.Е., Петросян В.И., Синицын Н.И. //Биомедицинская радиоэлектроника, 2000, No 2, с. 18.Петросян В.И., Синицын Н.И., Елкин В.А. //Наукоемкие технологии, 2000, No2, с.33-37

A NEW APPROACH AND RATIONALE

THE POSSIBLE MECHANICAL DYNAMICS OF BIOGENIC NITRIC OXIDE IN THE BODY

Douguchi V.V., Douguchi L. V.1

Russia, JSC “Atom-Med Center”, Moscow – Saint-Petersburg, GBOU VPO while doing researches them. And.

Without denying scientific advances in uncovering the mechanisms of formation and action of oxides of nitrogen in biological systems, on the basis of data interpretation of literary studies, personal observations presents a new mechanism of their formation and growth in vivo (under stress, HBO, hyperoxia, and others). Shows the physical characteristics of the elements and water structures of biological fluids, contributing to the processes of increase of nitrogen oxides.

MITOCHONDRIAL OXPHOS SYSTEM REARRANGEMENT CONTROLLED BY QUANTUM PROPERTIES OF BONDED WATER

Y.A. Skorobogatova1,2, S.V. Nesterov1,2, I.S. Litvinov1, L.S Yaguzhinsky1,2 1A.N. Belozersky Institute Of Physico-Chemical Biology MSU2Moscow Institute of Physics and Technology, E-mail: [email protected]

Литература

  1. Krasinskaya I.P et al. // FEBS Lett. 1984. V. 167(1) PP. 176-80.
  2. Krasinskaya I.P. et al. // Biochemistry. 1989. T. 54. PP. 1550-1556.
  3. Picas L. et al. // J Fluoresc. 2007. V. 17 PP. 649-654.
  4. Artmann G.M. et al. // Biophys. J. 1998. V. 75, P. 3179.
  5. Artmann G. M. et al. // Eur. Biophys. J. 2009. V. 38. P. 589.
  6. Malomuzh N.P., Oleinik A.V. // Journal of Structural Chemistry. 2008. V. 49(6). PP. 1055-1063.
  7. Bunkin A.F., Pershin S.M. // Bulletin of the Russian Academy of Sciences. Physics of Vibrations (BRAS).1997. V. 61(3). PP.158-164.
  8. Stebnovsky S.V. // Technical Physics. (Russian) 2002. V. 72(11) PP. 24-27.
  9. Pershin S.M. // Physics of Wave Phenomena. 2008, Vol. 16(1), PP. 15–25.
  10. TikhonovV.I.,VolkovA.A.//Science.2002V.296P.28.
  11. PershinS.M.,BunkinA.F.//LaserPhysics.2009.V.19(7).PP.1410-14.
  12. PotekhinS.A. et al. // Biophysical Chemistry 2005. V. 118 PP. 84 – 87.
  13. HalestrapAP//BiochemSocTrans.1994.V.22(2)PP.522-9.
  14. Orlov S.N. et al. // J Membr Biol. 1989. V. 107(2) PP. 105-17.

ULTRA-LOW DOSES OF NITROSOMETHYLUREA DECREASES INHIBITORY EFFECT OF FOLLICULAR FLUID ON REINITIATION OF MEIOSIS IN OOCYTE OF BOS TAURUS IN VITROD.N.Tatarskaya, T.I. Kuzmina 1, I.Ya.Shakhtamirov 2, H.M.Mutieva 2, T.I.Stanislavovich 1
Institute of Perinatology and pediatrics of Federal Almazov Medical Research Centre
1Russian Research Institute of Farm Animal Genetics and Breeding, e-mail: [email protected] State University

Литература

  1. Никифоров А.В., Подгурская А.Д. // Действие некоторых супермутагенов на организм животных. В сб.,Применение химических мутагенов в сельском хозяйстве и медицине. М., Наука, 1973, стр. 283.
  2. Кузьмина Т.И., Новичкова Д.А. // Научные труды YI Международного Конгресса «Слабые и сверхслабые поля и излучения в биологии и медицине», Санкт-Петербург, 2012, c.79.
  1. Кузьмина, Т.И., В.А. Багиров, А.В. Егиазарян, Х.Альм, Х.Торнер // Биотехнология получения эмбрионов крупного рогатого скота in vitro. Санкт-Петербург-Пушкин, 2009, 44 С.
  2. Tarkowski A.K. // Cytogenetic. – 1966. – V.1. – P. 394-400.

This work is supported by RFBR, grant No 15-02-08703.

ON THE CRITERIA FOR WEAK AND STRONG SIGNALS
IN A POLARIZATION-OPTICAL RESEARCH
Ya. A. Fofanov
Institute for Analytical Instrumentation, RAS, E-mail: [email protected]

At the present time is becoming clear the need for criteria to numerically characterize the various types of weak and ultra-weak fields and radiation, and other phenomena that are discussed at LFBM Congress. In this report the criteria are considered for weak and strong signals (responses) at highly sensitive polarization-optical studies of anisotropic and ordered objects and systems.

This work is supported by RFBR, grant No 15-02-08703.

Литература

  1. 1.Галль Л.Н. Физ. принципы функц. материи живого орг-ма. – Спб.: Изд-во Политехн. ун-та, 2014.
  2. Acher O., Bigan E., Drevillon B. Rev. Sci. Instrum. 1989. V. 60. No. 1. P. 65-77.
  3. Fofanov Ya. A. Proc. SPIE. 1992. V. 1811. P. 413-414.
  4. Фофанов Я.А., Плешаков И.В., Кузьмин Ю.И. Оптический журнал. 2013 г. Т. 80. No 1. С. 88-93.

OSCILLATIONS OF DYNAMICAL PARAMETERS OF STRUCTURISATION OF DRYING DROPS WITHDRAWING FROM THE BULK OF MULTICOMPONENT AQUEOUS SOLUTIONS AT REGULAR INTERVALS

T.A. Yakhno, A.G. Sanin
Institute of Applied Physics of Russian Academy of Science,
46 Ulyanov Street, Nizhny Novgorod, 603950, Russia. E-mail: [email protected]

Литература

  1. С.Э. Шноль, В.А. Коломбет, Н.П. Иванова, Т.Я. Брицина // Биофизика, 1980, т. 25, No 3, с. 409.
  2. В.А. Коломбет, Н.П. Иванова, Т.Я. Брицина, С.Э. Шноль // Биофизика, 1980, т. 25, No.2, с. 213.
  3. Т.А. Яхно, А.Г. Санин, C.V. Vacca, и соавт. // ЖТФ, 2009, т. 79, No10,с. 22.
  4. http://tea.jofo.ru/263654.html
  5. А.В.Дроздов, Нагорская Биофизика, 2014, т.59, No 6, с.1195, http://www.biophys.ru/archive/rhythm-00005.pdf
  1. ANTI-BUBBLES AS A PROTOTYPE OF LIVING CELLS
  2. Vladimir Shatalov
  3. Donetsk National University, Ukraine. E-mail: [email protected] central question in evolution is how simple proto-cells first arose and began the competitive process that drove

the evolution of life. Membranes are essential components of even the simplest cells. Membrane or lipid vesicle supports the inside vs outside asymmetry of concentration of various ions in a living cell. The gradients of concentrations are the driving forces of life processes. In this work we show that such vesicles with a lipid bilayer could be formed spontaneously from anti-bubbles in sea water in the surf zone.

An anti-bubble is a droplet of liquid surrounded by a thin film of gas. They are formed when liquid drops or flows turbulently into the same or another liquid contained a surfactant [1]. The molecules of the surfactant form two lipid layers on the air-water surfaces divided by thin air film. The hydrophobic tails of surfactant molecules are in opposition so the two lipid layers differ from the cell membrane just by the presence of the air film between the layers. The gravitational thinning of the air film result in it bursts because of van der Waals forces [2]. The published anti- bubbles lifetime [2] varies up to 10 min and more, depending on the surfactant, anti-bubbles size and salinity of water. We believe that at some conditions between these forces, surface tension and Zeta-potential of the interfacial layer the air film may be removed without damaging the two lipid layers. The process produces somewhat like lipid vesicle.

By solving diffusion equations we show that during the vesicle formation the concentration of inner ions goes down because these are partially adsorbed at the electric double layer of the membrane. Then we have to solve the boundary problem arising from the previous step to evaluate the steady state in the vesicle. We take into account that the absorption and transparency of membrane for Na+ ions differs from K+ ones due to their different sizes and masses. Finally, we obtain a spherical vesicle with lipid bilayer and Na+ vs K+ asymmetry that may be a prototype of a living cell. Sea water contains all components needed and surf repeats the experiment for ages.

References

1. 2. A. Tufaile, J. C. Sartorelli, Phys. Rev. E, 66 (2002), p. 056204(7). S. Dorbolo, et al., Europhys. Lett., 69 (2005), p. 966–970.

LOW INTENSITY ELECTROMAGNETIC FIELDS PRODUCE LOCAL THERMAL SPIKES IN BIOLOGICAL SYSTEMS

Vladimir Shatalov

Donetsk National University, Ukraine, E-mail: [email protected]

Traditionally, the effects of electromagnetic fields (EMF) on living organism are divided into ionizing (non- ionizing) and thermal (non-thermal). They are extensively investigated to date [1]. One well-understood effect of microwave radiation is dielectric heating, in which any polar solution (such as living tissue) is heated by rotations of the polar molecules induced by the electromagnetic field. In the case of a person using a cell phone, most of the heating effect occurs at the surface of the head, causing its temperature to increase by a fraction of a degree. The maximum power output from a mobile phone is regulated by the mobile phone standard and by the regulatory agencies in each country. In the USA, the Federal Communications Commission (FCC) has set a Specific Absorption Rate (SAR) limit of 1.6 W/kg, averaged over a volume of 1 gram of tissue, for the head. In Europe, the limit is 2 W/kg, averaged over a volume of 10 grams of tissue. SAR values are heavily dependent on the size of the averaging volume.

However, when considering effects of weak EMF, an important problem is to find and validate clear criteria of weakness of the impact [2, 3]. For thermal effects of the EMF the presently accepted criteria are based upon application of physiologically acceptable limits to the increase of the average temperature of the samples.

Non-thermal effects are not connected to the temperature increase, which is usually assumed to be less than 0.01 degrees C (living organism), 0.001 (cells) or 0.0005 (sub-cellular). By comparison, ANSI, WHO, IEEE & ICNIRP consider that exposures below 0.05 degrees C (0.4 W/kg) are safe for workers, and exposures below 0.01 C (0.08 W/kg) are negligible for the public. Any biological effects below these levels of heating are considered by these organizations to have no biological significance and to be reversible. These criteria are used for estimation of maximum permissible power of domestic radio equipment (cell phones, Bluetooth, Wi-Fi, etc.). The effect of mobile phone radiation on human health is the subject of great interest and numerous studies due to the enormous increase in mobile phone usage throughout the world. Also any biological effect of environmental RF EMFs is usually referred as non-thermal. Non- thermal effects can also arise due to the low-frequency pulsing of the mobile phones carrier signal. Biological significance of these modulations is subject to numerous debates. Another application of these effects lies with the development of non-lethal microwave weaponry (such as «Active Denial System», developed by the U.S. Department of Defense) to evaluate whether their effects are indeed reversible.

We believe that SAR limits are not universal and present estimations of the local thermal effects of the low EMF. The last takes into account inhomogeneity of the heating (resulting from non-uniformity of living tissue). The idea of the non-uniform heating, of course, is not new. However, that is hard to find specific estimates; therefore it was suggested by Challis [2] as an open problem. Our main goal is to provide such analytical estimations and give physical background of thermal effects related to inhomogeneity in space and time. We show that in some cases, the local temperature rise significantly outpaces ones given by the limits for the average absorbed radiation power and therefore the Watt to Weight SAR limits may be insufficient. The possibility of such a local heating (with the negligible average temperature increase) challenges the common division of EMF effects into thermal and non-thermal. While the effects, connected to the local heating, are thermal, the average heating may be negligible, making them classified conventionally as non-thermal.

Our consideration is not limited to any particular frequency band of the electromagnetic field. It is only important that the field is heating the conducting medium locally, be it via the eddy currents in the medium of non- uniform conductance, the polarization-induced vibrations of the molecules in the medium of non-uniform polarizability, or any other local mechanism. There are several different mechanisms, which lead to RF power absorption and have different penetration depths in different media.

It is obvious that the temperature distribution in any bounded sample heated by penetrated radiation has to be inhomogeneous. Firstly, it is due to the spatially inhomogeneous heating by the exponentially decaying RF power. It is well known that the temperature distribution has maximum under the sample surface if the heat transfer inside exceeds that one outside the sample (for example, due to pure transparency of the sample surface). The maximum value is limited by RF power decay depth that actually varies from fractions of a millimeter to several centimeters. Therefore, this maximum is flat and does not exceed much the surface temperature value. Unlike the case described, we will examine the temperature peaks at the micro inclusions having high electrical conductivity, which are located far enough away from the cooling surfaces or heat sinks.

It is well known that living tissue is substantially heterogeneous on micro and nano-scales with some of its components having increased electrical conductivity. Since the absorbed microwave and RF power is proportional to the electrical conductivity, conducting inclusions are heated by radiation more intensively than the surrounding media. To describe this process of non-uniform heating in the medium with thermal regulation (present in all living tissue on all scales) let us use the classical Pennes’ differential equation. It assumes that the total energy exchange between tissue and the flowing blood can be modeled as a non-directional heat outlet, whose magnitude is proportional to the volumetric blood flow and the difference between local tissue and major supply arterial temperatures. This approximation is valid for large enough samples, containing numerous blood vessels. To investigate the temperature distribution at a smaller scale, we explore a different model, in which a heated center indirectly interacts with the cooling surface. Such a model might describe the temperature distribution in a close vicinity of a blood vessel that we refer as a thermostat. To simplify the consideration, the heat absorbed inclusion is modeled as a sphere. Starting from such a spherical horse, we have explained the origin of temperature spikes in nano-inclusions contained in any tissue of any form. There exist two reasons giving temperature increase in a little inclusion. First is the increased heat production, and second – the remote distance from the cooling boundary. Another source of the temperature non-homogeneity is time inhomogeneous heating, so we explore the non-stationary case for the spherical model.

page117image2702303600

The results of our calculations of the relative temperature dynamics in a sample irradiated by a single RF pulse with width τw = 0.2τ, where τ is the heat diffusion time; for the size ratio x = 0.2 and the set of different heated power ratios y = 0, 1, 2, 4 and ∞ are shown in Fig. 1. In the case of dielectric inclusion in conducting media (y = 0) the temperature shift of media exceeds one of inclusion Θi /Θ0 < 1 only at initial moments, but soon after this ratio rapidly increases to some asymptotic value close to 3. For larger y the ratioΘi /Θ0 exceeds 1 at all times. For the conducting inclusion in dielectric media (y=∞) the relative temperature shift Θi /Θ0 is very high in initial moments because the media is heating only by the heat transfer outside from the inclusion. After the heating pulse finished all the curves go to the asymptotic value ~3.

The curves on Fig. 1 were calculated for the case of a single RF pulse but it is obvious if the time scale expands to a sequence of pulses then every next pulse will start at increased values of relative temperature of the inclusion and that amplifies the effect of inhomogeneous heating of the sample.

The results have to draw attention to the obvious and at the same time unexpectedly large spatial inhomogeneity of heating of the irradiated samples, regardless of the intensity of irradiation. Until now, it seemed to be obvious that RF power is the main criteria, which refers any RF exposure to the thermal or non-thermal one. However, we have shown that relative temperature rise in micro inclusions is RF power independent and may be very high because it is reversely proportional to the size of the inclusions. This is the case when the radiation is absorbed mainly in the inclusions that situated sufficiently far from the heat outlets. Moreover, qualitatively this conclusion is an obvious consequence from Fourier’s law and the heat transfer boundary conditions, and this is a long-solved problem in physics that leaves no questions.

To prove the results experimentally one has to measure the local temperature inside nano-objects that is not so easy but may be very informative. There exist many experimental works with inanimate samples, the results of which, we believe, can be interpreted in terms of local heating (or overheating) of irregularities in the irradiated samples. We believe that all these effects may be consequences of the inhomogeneous heating in micro- and nano-scales. There exists a lot of contradictory evidence for non-thermal effects of electromagnetic radiation on living organisms [2-4]. According to our paper, many of the interpretations need to be revisable. Now we can say that thermal mechanisms have to be favorable in explanation of any effect of MW on neurochemistry. Concerning discussions in Internet on safety of the non-lethal microwave weapon, it should be pointed to possible uncontrolled temperature rise in under skin inclusions. So, the local temperature can rise significantly outpaces the averaged one and therefore the Watt to Weight SAR limits may be insufficient to account the safety of RF radiation and the conventional division of the biological effects of electromagnetic fields on the thermal and non-thermal needs to be revised.

References

  1. NIEHS, 1999. Report on health effects from exposure to power-line frequency electric and magnetic fields. National Institute of Environmental Health Sciences of the U.S. National Institutes of Health. NIH Publication No. 99-4493.
  2. Challis, L.J., 2005. Mechanisms for interaction between RF fields and biological tissue. Bioelectromagnetics, Supplement 7, 98-106.
  3. Shatalov, V.M., 2012. Mechanism of the biological impact of weak electromagnetic fields and the in vitro effects of blood degassing. Biophysics, 57, 808-813.  BIOLOGYCAL EFFECTS OF LOW-INTENSITY EXTREMELY HIGH FREQUENCY ELECTROMAGNETIC RADIATION ON SACCHAROMYCES CEREVISIAEZelena L.B., Gretsky I.A., Kachur T.L.

Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Zabolotnogo str., 154, Kyiv 03680, Ukraine, E-mail: [email protected]

Today results of many experimental studies demonstrate the biological effects determined by extremely high frequency electromagnetic radiation (EHF EMR) that is used in medical practice. Despite the lack of reliable theoretical mechanisms, the basic laws of interaction between EHF EMR and biological objects have been formulated and promoted further researches in this area. It was postulated that EHF EMR effects are defined by its biotropic parameters (intensity, frequency, exposure, etc.) and the nature of the biological object.

In the present study influence of low-intensity EHF EMR on Saccharomyces cerevisiae cell-cell interaction process and genome stability has been assessed. Yeast cell suspension was exposed to EHF EMR (carrier frequency of 61.2 GHz, incident power density of 50 μW/cm2) for 30, 60 and 90 min. As EHF EMR source we used serial MRTA-02 with horn antenna (S = 2 cm2), working in a mode of continuous generation.

Yeast flocculation is defined as an asexual aggregation process of yeast cells into clumps with subsequent fast sedimentation in the medium in which they are suspended [1]. There are several hypotheses to explain the mechanism of flocculation: they consider biochemical, molecular, genetic characteristics of the process of cell-cell interaction [2]. Flocculation ability of S. cerevisiae was measured in standard conditions, using a microflocculation technique described previously [3]. Results of analysis revealed that yeast cells subjected to EHF EMR showed a slight flocculation rising up to 15% while the exposure duration was increasing. It might be assumed change of cell wall charge caused by EHF EMR.

To examine yeast genome variability that can be induced by EHF EMR of various durations (30, 60 and 90 min), amplification with primers to di- and tetranucleotide repeats was performed. PCR with mini- and microsatellite primers is useful tool for fast genome analysis without knowing nucleotide sequences. Such type of DNA-markers is widely used to differentiate and identify yeast species and strains [4]. Analysis of amplicon patterns revealed that the size of amplification products varied from 300 to 2000 bp and there were no differences between PCR-fragments of non- irradiated and irradiated samples.

Thus, results obtained in our study suggest that EHF EMR did not affect S. cerevisiae genome but facilitated cell-cell interaction and yeast flocculation.

SUPERWEAK INFLUENCES IN ASTROPHYSICS AND GEOPHYSICS

B.M.

Superweak influences might be determined as such influences, when the energy introduced into the system is in several orders lesser than the energy connected with the kinetics of the system itself. This type influence is observed not only in molecular level. Two examples art presented: the planets affect upon solar activity variations; solar activity influence on climate subsystem of the Earth.

Литература

1. Галль Л.Н., В мире сверхслабых, СПБ, 2009, 317 с.
2. Горшков М.М., Солнце, планеты и биосфера, Биофизика, т. 37, No 4, 1992, с. 805 – 816.
3. Котов В.А., Сорок лет измерений общего магнитного поля Солнца. Взгляд из сегодня, Известия

Крымской астрофиз. обсерватории, т. 105, No 1, 2009, с. 75 – 90.
4. Панкратов А.К. и др., К вопросу о резонансных свойствах солнечной системы, Известия Крымской астрофиз. обсерватории, т. 93,1996, с. 53 – 57.
5. Wolff C.L., Patrone P.N., A new way that Plakets affect the Sun, Solar Physics, v. 266, 2010, pp. 227 – 246.
6. Наговицин Ю.А., Огурцов М.Г Труды международной конференции «Климатические и экологические аспекты солнечной активности», СПБ, 2003, с. 321 – 326.

THE CHRONORYTHMOLOGIC ASPECTS OF ATORVASTATIN RENOPROTECTIVE ACTION

Zamorskii I. I., Zeleniuk V. G.
Bukovinian State Medical University, 2, Teatralna sq., Chernovtsy, 58002, Ukraine; E-mail: [email protected]

The effect of atorvastatin at a dose of 20 mg/kg on some parameters of the renal function in rats with acute renal failure depending on the time of drug administration was studied. Rhabdomyolitic ARF was modeled by intramuscular injection of 50% glycerol solution at a dose of 10 ml/kg. As has been found in our experiments, atorvastatin increases diuresis in animals with ARF with maximal efficacy at 08.00 and minimal efficacy at 20.00. Herewith, the glomerular filtration rate (GFR) mesor increases by 39% (р<0,05) with the antiphase character of the rhythm of proteinuria. The experimental data indicate dependence of the renoroprotective action of atorvastatin on the time of its administration: maximum increase in diuresis, glomerular filtration rate and decrease in proteinuria fall on the night and morning periods of the day.

Литература

  1. Zamorskii I.I., Zeleniuk V.G. Renoprotective effects of statins under the conditions of acute renal failure, caused by rhabdomyolysis // Biophysics. 2014. V. 59, N. 5. P. 837–840.
  2. Stow L. R., Gumz M. L. The Circadian Clock in the Kidney // J. Am. Soc. Nephrol. 2011. V. 22. N. 4. P. 598– 604.
  3. Брюханов В. М., Зверева А. Я. Роль почки в регуляции суточных ритмов организма // Нефрология. 2010. Т. 14. No 3. С. 17–31.

SPECIFIC FEATURES OF PALYNOTERATICAL “ANSWERS” OF PHYTOCENOSIS ON CHERNOBYL CATASTROPHE

G.M. Levkovskaya, V.P. Matzko1, Skvernyuk I.I.1, Orekhova M.G.1
Institute for the History of Material Culture of the Russian Academy of Sciences, E-mail: [email protected];1 Institute of Radiobiology of the National Academy of Sciences of Belarus

Литература

  1. Levkovskaya G.M. Palynoteratical Сomplexes as Indicators of the Ecological Stress, Past and Present // Proceedings of the 5th European Palaeobotanical and Palynological Conference. Acta Palaeobotanica. International Journal of Palaeobotany and Palynology. Suppl. 2. Kraków, 1999, p. 643 – 648.
  2. Левковская Г.М, Боголюбова А.Н. // Материалы XI Всероссийской палинологической конференции«Палинология: теория и практика». М. ПИН РАН, 2005, с. 132 – 133.
  3. Левковская Г.М., Боголюбова А.Н. Качество пыльцы и особенности палинокомплексов некоторых природных экстремумов плейстоцена и их сравнение с палинокомплексами отложений с высоким радиоактивным заражением из района чернобыльской техногенной катастрофы // Проблемы современной палинологии. Мат. XIII Российской палинол. конф. Т. 2. Сыктывкар. ИГ Коми НЦ УрО РАН, 2011, с. 278-285.
  4. Levkovskaya G.M. Pollen indication of the natural and Chernobyl type geobotanical catastrophes // Japanese Journal of Palynology. Abstracts. 13th International Palynological Congress. 9th International Organization of Palaeobotany Conference. Tokyo, 2012, No58, p. 126-127.

CONDITION OF THE MYOCARDIUM OF VOLUNTEERS AT VARIOUS LATITUDES AT CHANGE OF PARAMETERS OF SPACE WEATHER

Samsonov S.N., Parshina S.S.1 , Manykina V. I., Petrova of P.G.2 , Strekalovsky A.A.2 , Palshina A.M.2 , Afanasyeva T.N.1 , Petrova V.D.1 , Kaplanova T.I.1, Potapova M.V.1

Yu.G.Shafer Institute of Cosmophysical Research and Aeronomy of Siberian Branch of the Russian Academy of Sciences, Russia, Yakutsk, [email protected]
1 Saratov State Medical University n.a. B.I. Razumovsky, of the Russian Ministry of Health, Saratov
2 Medical Institute of North-Eastern Federal University of M. K. Ammosov, Russia, Yakutsk

Research of reaction of a myocardium at change of parameters of space weather is an actual task as cardiovascular pathology mortality is still in the lead in structure of the general mortality. Changes of intensity of a magnetic field of Earth as a result of active processes on the Sun and in a magnetosphere of Earth are one of the most important factors of possible failure of adaptation and an exacerbation of chronic diseases. The researches on influence of parameters of space weather conducted in recent years showed that the healthy person on Earth without serious consequences can transfer such influence. At the same time impact of the disturbance parameters of space weather on the human being with the weakened adaptation can lead to serious complications, up to a lethal outcome. For this reason detection of features of adaptation of cardiovascular system of the healthy person to the changing conditions of a geomagnetic disturbance will allow to develop further a number of preventive measures within preventive cardiology. It is known that values of a geomagnetic disturbance differ depending on supervision place width. Therefore authors assumed, as impact on cardiovascular system has to depend on location of the human being. For the purpose of identification of impact of space weather on health of the human being at various latitudes experiment within the international “Heliomed» project with groups of volunteers on the aurora (settlement of Tixie), the subauroral (Yakutsk) and middle latitudes (Saratov) was made. In work differences in influence of parameters of space weather on a condition of cardiovascular system of volunteers at various latitudes are shown.

NOVEL DATA ON BACTERIAL EFFECTS OF LOW INTENSITY ELECTROMAGNETIC FIELD IN EXTREMELY HIGH FREQUENCES:
EFFECTS OF IRRADIATED ANTIBIOTICS ON ENTEROCOCCUS HIRAE AND ESCHERICHIA COLIGROWTH AND MORPHOLOGY. ION FLUX ALTERNATIONS DUE TO COMBINED TREATMENT OF LACTOBACILLUS ACIDOPHILUS WITH IRRADIATION AND ANTIBIOTICS

Ohanyan V.1, Torgomyan H.1, Soghomonyan D2, Hovnanyan K.3, Trchounian A. 2

1Department of Biophysics and 2Department of Microbiology, Plants and Microbes Biotechnology, Faculty of Biology, Yerevan State University, 0025 Yerevan, Republic of Armenia 0025, E-mail: [email protected]
3Institute of Molecular Biology of the National Academy of Sciences of the Republic of Armenia, 0014 Yerevan, Armenia

The electromagnetic irradiation of natural and artificial origins is very interesting from scientific and industrial point of view. Electromagnetic fields (EF) in extremely high frequencies (EHF) in the range of 30 GHz to 300 GHz with low intensity especially were discovered to affect biological objects [1]. From our research perspective to reveal novel effects and to study appropriate mechanisms, we have selected three wild type strains of different bacterial species: Enterococcus hirae ATCC 9790, Lactobacillus acidophilus VKMB-1660, Escherichia coli K12.

E. hirae and E. coli were treated with irradiated (the frequency of 51.8 or 53 GHz; the EF flux capacity of 0.06 mW/cm2) antibiotic ceftazidime, while L. acidophilus was treated with non-irradiated ceftazidime, due to research goals. Interestingly effects of irradiated ceftazidime are more noticeable, and decrease specific growth rate by > ~12% differs from non-irradiated ceftazidime when E-coli is used, while in the same conditions the growth depressive effects are similar, if E. hirae is used [2]. In order to reveal possible mechanisms, both bacterial strains were photo documented using scanning electron microscope (SEM) of TESCAN (Czech Republic) and X-ray micro-analyzed. Based on results, we have discovered that irradiated with 53GHz EF in EHF coherent waves for 1 h bacterial cells of E. coli increase their volumes ~60%, while E. hirae cells swell ~14%. The mentioned difference may be due to membrane dissimilarities, as long as E. coli is Gram-negative, while E. hirae is Gram-positive bacteria. The difference in response to irradiation may explain the level of susceptibility of these bacterial strains.

The second aspect of our research was investigation of effects of EF in EHF on L. acidophilus. The latter is one of the most known human gut probiotic lactic acid bacteria. Nowadays there are many physical factors and chemicals which can affect gut microbiota and lead to disturbance of their functional activity. As shown before in our laboratory, the low intensity EF at the frequencies of 51.8 and 53 GHz combined with antibiotic ceftazidime suppressed the growth and survival of L. acidophilus [3]. The aim was to study effects of EF irradiation and ceftazidime on energy-dependent H+/K+ exchange by L. acidophilus for revealing the mechanisms these effects. The bacteria were irradiated with two frequencies for 1 h and then if necessary treated with ceftazidime (20 μМ) and/or N,N’-dicyclohexilcarbodiimide (DCCD) (0.2 mM) for 10 min. H+/K+ exchange was determined using selective electrodes.

It was shown that upon glucose (22 mM) supplementation the medium acidification and K+ accumulation by bacteria were observed. In comparison with control, the H+/K+ exchange fluxes of irradiated at two frequencies samples were suppressed in approx. 25 %. The combination of irradiation and antibiotic inhibited the ion fluxes in approx. 35%. It is interesting that irradiation and antibiotic increased the inhibition the DCCD-sensitive H+/K+ exchange fluxes to approx. 80 %. Taken together with data on growth and survival [3], it was suggested that H+/K+ exchanging membrane proteins of L. acidophilus might be a target for extremely high frequency electromagnetic irradiation and antibiotics.

These effects of EF in EHF on growth, morphology and membrane-associated activity of different bacteria and their combination with antibiotics are of significance to apply in biotechnology, food industry and to employ in biosafety

CORRELATION ANALYSIS OF PHYSIOLOGICAL PARAMETERS, THEIR REACTION TO SMALL DOSES

Egorov V. V.
Moscow Skryabin state Academy of veterinary medicine and biotechnology, Skryabin str., 23. [email protected]

In work for humans and dogs (sample 50 and 10, respectively) obtained dependence of systolic, diastolic blood pressure and pulse rate from the temperature of the body in the form of “angular dependencies”. Switching the mode of operation of the cardiovascular system is due to the action of baroreceptors. The coordinates of the fracture are characteristic of an organism at a given time, as evidenced by the presence of a linear rising (pressure) and descending (temperature) dependencies from their age, known from the literature.

Correlation of physiological parameters can be used for diagnosis. It is established that the presence of the splitting line graph “Psystolic – Pdiastolic” on some straight pathologies of the cardiovascular system. Physiological parameters are sensitive to ultra-weak stress, for example, the food and drug in vitro. This allows you to pick up a sick diet and medicines.

THE TIES BETWEEN THE INFRADIAN RHYTHMIC OF THE PHYSIOLOGICAL PROCESSES IN AN ORGANISM WITH THE HELIOGEOPHYSICAL VARIATIONS UNDER THE INFLUENCE OF THE LOW INTENSIVE ELECTROMAGNETIC MICROWAVE RADIATION
Chuyan E.N.
«V.I. Vernadsky Crimean Federal University» Tavria Academy, Simferopol, Russia, e-mail: [email protected]

This work is dedicated to the searching for the relationship of the infradian rhythmic of the physiological processes in an organism under the influence of the artificial low intensity microwaves of the extremely high frequency, with the heliogeophysical variations. It was found that the essence of the ties between the physiological processes and the heliogeophysical variations is the synchronization of the organism biorhythms by the low intensity microwaves. The sporadic enhancement of the microwave emission of the Earth ionosphere during the Solar flares and the magnetic disturbances have influence on an organism state, and its correction may be provided by the low intensity extra high frequency microwaves.

RESPONSE OF PHOTOBACTERIUM PHOSPHOREUM LUMINESCENCE TO LOW-INTENSITY EXTREMELY HIGH-FREQUENCY ELECTROMAGNETIC RADIATION

Gretsky I.A., Gromozova E.N.

D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Zabolotnogo str., 154, Kyiv 03680, Ukraine, E-mail: [email protected]

The dramatic rise in electromagnetic radiation (EMR) levels is contributing to the increasing anthropogenic stress. One of the important research areas is the study of unicellular organisms response to extremely high frequency EMR (EHF EMR). This is due to the fact that this band is absent in the EMR natural spectrum and therefore there were not adaptations evolved to its action in living organisms [1]. The mechanisms of low-intensity EHF EMR biological effect are poorly understood.

Luminous bacterium Photobacterium phosphoreum was used as biological indicator for description of EHF EMR status. Due to high sensitivity and response speed, simplicity and efficiency bioassays based on luminescent bacteria have been widely used in research for rapid monitoring of EHF EMR effects [1]. The aim of our research was to study the EHF EMR irradiation effect on P. phosphoreum IMV B-7071 luminescence.

The bacterial suspension (V = 3 ml, 107 cells/ml) in minitubes was exposed to EHF EMR irradiation. We used commercial device MRTA-02 with horn antenna (S = 2 cm2), working in a mode of continuous generation (carrier frequency of 61.2 GHz, power flux density of 50 μW/cm2) as a EHF EMR source. Bacterial cell suspension was exposed to EHF EMR for 5, 15 and 30 min. EHF EMR effect was assessed by the change in the intensity of luminescence. Experimental luminometer setup based on photomultiplier tube (FEU-115M, 1400 V) was used to register the bioluminescence intensity.

The results of the impact of EHF EMR on bacteria luminescence showed the nonlinear dependence of the luminescence intensity on duration of exposure. The 5 min – influence of EHF EMR on bacterial cell led to an increase in luminescence intensity by (21,3±6,4)% in comparison with control. Upon further EHF EMR irradiation after 15 minutes from the start it was observed an increase in bioluminescence by (41,7±5,9)%. More substantial changes of bacterial luminescence were marked after exposure to EMR for 30 min. In this case the level of bioluminescence increased by (73,9±8,2) %.

Thus, these results suggested the perspectives of P. phosphoreum IMV B – 7071 use as an indicator of EHF EMR action with potential of the development of biosensor device.

TERAHERTZ RADIATION AT NITROGEN OXIDE FREQUENCIES IN TREATMENT OF PATIENTS WITH ANGINA
S.S. Parshina, T.N. Afanasjeva, А.V. Vodolagin. V.D. Tupikin1
Saratov State Medical University n.a. B.I. Razumovsky, of the Russian Ministry of Health, Saratov, [email protected]
1 State University named after N.G. Chernyshevsky, Saratov

Efficiency of terahertz therapy at frequencies of a molecular range of nitrogen oxide (150,176 … 150,664 GHz) (EMR THF or THF-NO-therapy) at patients with angina is analysed. It is established that the stablest effect THF-NO- therapy is the antianginal effect which doesn’t depend on the form of angina form (stable, unstable), a sex, age, existence or lack of the fact of smoking, and also a type of the faltering mode of influence. The sensitivity of haemodynamics, rheology and blood coagulation in to THF-rays is different in certain groups. THF-NO-therapy stops the phenomena of a chronic disseminirovanny intra vascular fibrillation at patients with heavy stenocardia of tension.

ASSESSMENT OF THE HUMAN BODY MAGNETICALLY OF GAS DISCHARGE VISUALIZATIONYu. Rybakov
Federal research center for projects evaluation and consulting services, E-mail: [email protected]

Литература

1. Коротков К.Г. //Основы ГРВ биоэлетрографии. С-Пб, изд. ИТМО, 2001.

LONG-TIME LIGTH-HOLOGRAPHIC TESTING OF HUMAN PSYCHOPHYSIOLOGICAL STATE

Trofimov A., Serdyukov D., Sevostyanova E.
International Scientific-Research. Institute of Cosmic Anthropoecology, Russia,630090, Novosibirsk, Lavrenieva, 6/1, [email protected]

Литература

  1. Trofimov A. The Problem of Helio-Gerontology. The New Scientific Point of View at the Processes of Aging and Water-Holographic Prevention. J. of US-China Med. Science, 2013, V. 10, 3–4, P. 63–70.

Full text Low and Superlow Congress 7, 2015, here (mostly in Russian):

http://biophys.ru/archive/congress2015.pdf